Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
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psd3 is a variant of the photosensitive degron built around an AsLOV2 photoreceptor module for light-dependent control of protein abundance. Available evidence indicates that its light sensitivity can be tuned by photocycle mutations and that it was developed in a system for optogenetic regulation of transcription-related processes.
The photosensitive transcription factor (psTF) is an AsLOV2-based optogenetic multi-component switch for light-regulated control of transcription. In the cited work, photocycle mutations in AsLOV2 were used to tune the light sensitivity of psTF photoreceptor components, and distinct AsLOV2 variants showed context-dependent behavior.