Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

4 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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photosensitized singlet oxygen generation

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KillerRed

Protein Domain
Since 2020photodynamic receptor activationphotosensitized singlet oxygen generationSelection / Enrichment

KillerRed is a genetically encoded protein photosensitizer used to generate singlet oxygen upon light irradiation for photodynamic perturbation. In the cited study, plasma-membrane-targeted KillerRed activated cholecystokinin 1 receptor (CCK1R) signaling in AR4-2J cells, producing persistent calcium oscillations consistent with permanent photodynamic receptor activation.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59

miniSOG2

Protein Domain
Since 2020photodynamic receptor activationphotosensitized singlet oxygen generation

miniSOG2 is a genetically encoded protein photosensitizer used as a light-responsive domain for photodynamic signaling perturbation. In AR4-2J cells, plasma-membrane-targeted miniSOG2 triggered persistent calcium oscillations upon light irradiation, consistent with permanent photodynamic activation of cholecystokinin 1 receptor (CCK1R).

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59

SOPP

Protein Domain
Since 2020photodynamic receptor activationphotosensitized singlet oxygen generation

SOPP, the singlet oxygen protein photosensitizer, is a genetically encoded protein-domain photosensitizer activated by light. In the cited 2020 Biomolecules study, plasma-membrane-targeted SOPP in AR4-2J cells triggered persistent calcium oscillations upon irradiation, consistent with photodynamic activation of cholecystokinin 1 receptor (CCK1R).

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59

genetically encoded singlet oxygen photosensitizing protein

Construct Pattern
Since 2019light-controlled reactive oxygen species productionphotosensitized singlet oxygen generationsubcellular localization targeting

Using a genetically encoded protein-encased photosensitizer, one could produce a given ROS with a controllable yield in a specific intracellular domain or compartment. A palette of ROS sensitizing protein derivatives has emerged and, in this review, we use information gleaned from recent studies to discuss properties that define a 'good' singlet oxygen photosensitizing protein.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59
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