Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
11 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-11 of 11
our optimized prime editing strategy provides a highly efficient and versatile framework for genome engineering in vitro
Here, we develop an ultra-efficient prime editing (UtPE) system for dicots by integrating evolved PE6 variants (PE6c and PE6ec), an altered pegRNA (aepegRNA), an RNA chaperone, and a geminiviral replicon.
Split pegRNA prime editors (SnPEs) are prime editing constructs in which a modified pegRNA is divided into an sgRNA and a separate prime RNA. In the cited 2022 study, this split architecture maintained prime editing activity while increasing design flexibility.
Tethered prime editors (tPEs) are prime editing constructs in which stem-loop aptamer-modified pegRNAs are tethered to Cas9 nickase. The design alters pegRNA architecture to increase prime editing efficiency and flexibility.
UtPE significantly improves editing performance in tomatoes, with UtPEv1 excelling in simple edits (unstructured RTTs).
UtPE significantly improves editing performance in tomatoes, with UtPEv3 effective for complex targets (structured RTTs or multiple nucleotide changes).
Compact PE is a truncated prime editor in which the reverse transcriptase RNase H domain is completely deleted. In the cited 2021 study, this compact PE showed editing activity comparable to full-length PE2 and enabled flexible split prime editor designs for in vivo delivery.
Split prime editor is a multi-component prime editing system in which prime editor is divided into two fragments to support flexible split configurations and dual-adeno-associated virus delivery. The reported design was enabled by a compact PE2 variant with complete deletion of the reverse transcriptase RNase H domain while retaining editing activity comparable to full-length prime editor.
In this study, a unique approach of F9 prime-editing was tested for the first time.
Here, we develop an ultra-efficient prime editing (UtPE) system for dicots by integrating evolved PE6 variants (PE6c and PE6ec), an altered pegRNA (aepegRNA), an RNA chaperone, and a geminiviral replicon.
Combining this error-suppressing strategy with the latest efficiency-boosting architecture, we design a next-generation prime editor (vPE). Compared with previous editors, vPE features comparable efficiency yet up to 60-fold lower indel errors, enabling edit:indel ratios as high as 543:1.