Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-5 of 5
Bacterial degrons are proteolysis-targeting signals that are appended to proteins to direct their degradation in bacterial cells. They are described as tools to interrogate and control protein function through targeted protein depletion.
In addition, we developed a light-inducible version of GFE3, paGFE3, using a novel photoactivatable complex based on the photocleavable protein PhoCl2c. paGFE3 degrades Gephyrin and ablates inhibitory synapses in response to 400 nm light.
The photo-N-degron is a peptide tag for optogenetic control of protein function in vivo through light-mediated protein degradation. It was reported to direct light-dependent degradation in Saccharomyces cerevisiae and Drosophila melanogaster, including light-dependent loss of Cactus function in developing Drosophila embryos.
Split-Cas9-based targeted gene editing is a gene-editing component used in an optogenetically coordinated platform with nanobody-mediated proteolysis-targeting chimeras. In the reported application, it was used to regulate Survivin as part of a multi-level strategy to control cancer cell fate.
Light-engineering technology, as described in the cited review, is an engineering approach that uses environmental light manipulation with light-emitting diodes (LEDs) to modulate plant defense. The review presents it as a potential strategy, alongside genome engineering, for fine-tuning crop defense and yield.