Toolkit Items

A stimulus triggers a structural change that exposes a previously hidden functional element.

A stimulus triggers targeted degradation of a protein via the proteasome or other pathways.

A protein binds DNA in a stimulus-dependent manner to regulate gene expression.

Two different proteins are brought together by a stimulus, enabling recruitment or complex formation.

A protein is recruited to a membrane surface (e.g. plasma membrane) by a stimulus.

A protein self-associates into multimers upon stimulation, enabling clustering or activation.

Light breaks a covalent bond, irreversibly releasing a caged peptide or domain.

A protein or RNA element binds RNA to control translation or stability.

Post-transcriptional regulation of mRNA translation rate or efficiency.

In silico design of protein sequences, structures, or circuits using algorithms or machine learning.

Iterative mutagenesis and selection to evolve improved biological parts.

Measuring the activity or performance of an engineered part in a biological context.

Experimental selection from a library to enrich for variants with desired properties.

Confirming construct identity by Sanger or next-generation sequencing.

Determining 3D structure via X-ray crystallography, cryo-EM, or NMR.