Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
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Inteins are internal protein domains that mediate conditional protein splicing as a post-translational control strategy. The supplied evidence describes switchable inteins as being developed to control splicing in ways compatible with applications in living cells.
The light-activatable gp41-1 intein is an engineered split intein system that enables blue light–dependent protein splicing to control Cre recombinase activity in mammalian cells. It is a multi-component switch derived from gp41-1 split variants engineered at unnatural split sites with reduced fragment affinity, allowing conditional reconstitution of Cre.
The light-activatable NrdJ-1 intein is a blue-light-gated split intein system engineered for regulated protein splicing in mammalian cells. In the reported study, it enabled blue light–dependent reconstitution of Cre recombinase activity and was used to drive recombination-linked outputs, including spatial control of apoptosis.