Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
SpyTag/SpyCatcher interaction is a protein-domain interaction used as an assistance strategy to improve split fluorescent proteins. In the cited 2019 Communications Biology study, it was demonstrated alongside directed evolution as an approach for improving split sfCherry-based reporters.
Caveolin interacts with eNOS through a region called the caveolin scaffolding domain (amino acids 82–101 of Cav1) and this interaction is sufficient to inhibit eNOS function in vivo.