Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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recruitment of the tandem sh2 domain of zap70 to clustered cd3ζ

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light-controlled optogenetic CD3ζ clustering tool

Multi-Component Switch
Since 2020cd3ζ phosphorylation-dependent signal initiationlight-induced spatial clusteringprotein clusteringrecruitment of the tandem sh2 domain of zap70 to clustered cd3ζrecruitment of zap70 tandem sh2 domains to clustered cd3ζsignal initiation through cd3ζ phosphorylation

The light-controlled optogenetic CD3ζ clustering tool is a multi-component optogenetic system engineered to induce spatial clustering of CD3ζ chains with light. In the cited 2020 study, light-driven CD3ζ clustering was sufficient to initiate proximal T cell receptor signaling, including CD3ζ phosphorylation and recruitment of the tandem SH2 domain of Zap70 to plasma membrane clusters.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 37
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