Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

3 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

Concrete methods used to design, build, verify, or characterize engineered systems.

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resonance energy transfer

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LOV2-based optogenetic actuators

Protein Domain

LOV2-based optogenetic actuators are light-responsive protein-domain tools whose switching can be sensitised and monitored in situ by resonance energy transfer. The available evidence supports their use as optogenetic actuators with resonance energy transfer integrated into the readout and sensitisation strategy.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59
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