Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

3 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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reversible photoactivation

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LOV2 domain from Avena sativa phototropin1

Protein Domain
Since 2012domain-fusion-mediated activity modulationlight-dependent allosteric switchingreversible photoactivation

The LOV2 domain from Avena sativa phototropin1 is a blue-light-responsive sensory domain that was fused to the restriction endonuclease PvuII to create a genetically encoded light-controllable chimeric nuclease. In this context, LOV2 modulates DNA cleavage activity in response to blue light, with the direction of regulation determined by the fusion interface.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59

blue-light-induced K(+) channel 1

Multi-Component Switch
Since 2015allosteric switching through lov2-jα fusionlight-gated channel openingmembrane hyperpolarization via potassium-selective conductancepotassium-selective conductancereversible photoactivation

BLINK1 is a blue-light-induced potassium channel engineered by fusing the plant LOV2-Jα photosensory module to the small viral K+ channel Kcv. It is reversibly photoactivated by blue light and channel opening drives membrane hyperpolarization toward the potassium equilibrium potential.

CFBacMamMusHumTxRep
Ev 36Rep 9Pr 37

LOV-PvuII fusion enzyme

Multi-Component Switch
Since 2012light-dependent allosteric switchingPhotocleavagereversible photoactivation

The LOV-PvuII fusion enzyme is a genetically encoded light-controllable endonuclease created by fusing the Avena sativa phototropin1 LOV2 photosensory domain to the restriction enzyme PvuII. In analyzed variants, blue light modulated DNA cleavage activity relative to dark conditions, with the direction of regulation determined by the fusion interface.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 37
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