Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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reversible photoswitching by red and far-red light

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synthetic transcription factor

Construct Pattern
Since 2015light-controlled nuclear localizationreversible photoswitching by red and far-red lighttranscriptional activation following nuclear translocation

The synthetic transcription factor is an engineered cargo used with a red/far-red light-regulated phytochrome-based nuclear localization system. In mammalian cells and zebrafish, its light-controlled nuclear translocation was used to drive transgene expression as a functional readout of regulated protein import.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
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