Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
7 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-7 of 7
Inducible RNAi depletion of CIB1 is a conditional gene-silencing method used in vitro and in vivo to reduce expression of calcium- and integrin-binding protein 1 (CIB1). In triple-negative breast cancer models, CIB1 knockdown impaired cell survival, altered transcriptional programs linked to reduced proliferation and increased cell death, and caused marked shrinkage of MDA-MB-468 xenograft tumors.
Small interfering RNA (siRNA) is a short RNA modality used to transiently downregulate expression of a target gene by exploiting the RNA interference pathway. In the supplied evidence, siRNA is described as a gene-therapy approach for altering gene expression rather than a genome-editing reagent.
Double-stranded RNA (dsRNA) was used as an RNA interference trigger in the Madeira cockroach to knock down the clock genes Rm-per, Rm-tim1, and Rm-cry2. Single injections produced successful and permanent reduction of the corresponding mRNAs within about two weeks and enabled functional analysis of circadian clock components.
shRNA delivered by lentivirus was used as an RNA interference-based perturbation method to reduce endogenous CIB1 levels in endothelial cells. In the cited study, this knockdown approach was applied to test CIB1 function in endothelial haptotaxis and tube formation.
Small interfering RNA modified at the 5' antisense phosphate is an siRNA variant evaluated as a potential light-responsive RNAi switch. Evidence from a 2007 Oligonucleotides study indicates that this modification is tolerated by the RNA interference machinery and still supports RNAi, although at lower activity than fully native siRNA.
Small interfering RNA with randomly incorporated photolabile groups is a chemically modified RNAi reagent whose gene-silencing activity can be modulated by light. Available evidence indicates that siRNA can retain RNA interference activity despite certain chemical modifications, including modification at the 5′ antisense phosphate, although activity is reduced relative to native siRNA.
Spray-induced gene silencing (SIGS), which applies externally produced dsRNA through foliar sprays, seed treatments, or root uptake, provides practical advantages over genetic modification, including rapid deployment, environmental compatibility, and target specificity.