Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
Short-hairpin RNA depletion of CIB1 is an RNA interference-based gene knockdown method used in HEK293 cells to reduce CIB1 expression. In the cited study, stable and transient CIB1 depletion increased cellular Ca2+ responses to the InsP3-generating ligands ATP, UTP, and carbachol.
The mice were treated by nasal inhalation of 2-OMe-modified HSPA12B siRNA... Nasal inhalation of nano-polymer-encapsulated HSPA12B siRNA specifically downregulated mRNA and protein expression levels of HSPA12B in lung tissues.
Lentivirus-mediated short hairpin RNA targeting Cib1 is a gene-silencing perturbation used to reduce CIB1 expression in mouse cardiac settings. In the cited study, intramuscular delivery of a lentiviral shRNA construct was used to examine how CIB1 downregulation affects myocardial fibrosis, cardiac dysfunction, and PI3K/Akt signaling after myocardial infarction or during angiotensin II stimulation.