Cj4Cas9
Construct PatternAmong these, Cj4Cas9 stood out as particularly noteworthy due to its compact genome size (985 amino acids) and unique PAM preference (5'-NNNGRY-3').
CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
Toolkit Items
Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
▾Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 1
Mechanisms
Layer 2
Architectures
▾Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 2
Architectures
Layer 3
Components
▾Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Layer 3
Components
Technique Branch
Layer 1
Approaches
▾High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 1
Approaches
Layer 2
Methods
▾Concrete methods used to design, build, verify, or characterize engineered systems.
Layer 2
Methods
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rna-guided dna cleavage
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enCj4Cas9
Construct PatternSeeking to further expand its utility, we engineered Cj4Cas9 for higher activity by introducing L58Y/D900K mutations, resulting in a variant termed enCj4Cas9. This variant exhibits a two-fold increase in nuclease activity compared to the wild-type Cj4Cas9 and recognizes a simplified N3GG PAM, considerably expanding its targeting scope.
CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
SpCas9 nucleases
Protein DomainSince 2017mutation-mediated reduction of cleavage without equivalent reduction in dna bindingPhotocleavagerna-guided dna cleavage
SpCas9 nucleases in this context are increased-fidelity CRISPR-Cas9 variants generated by combining mutations from eSpCas9 and SpCas9-HF1 into HeFSpCas9 forms. They are designed to preserve RNA-guided DNA cleavage while improving genome-editing specificity in a target-dependent manner.
CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
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