Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
Stem-loop prime editors (sPEs) are modified prime editing guide RNA constructs in which stem-loop aptamers are added to the 3′ terminus of the pegRNA. This altered pegRNA architecture is used as a basis for tethered prime editors and for split pegRNA prime editor configurations.
Tethered prime editors (tPEs) are prime editing constructs in which stem-loop aptamer-modified pegRNAs are tethered to Cas9 nickase. The design alters pegRNA architecture to increase prime editing efficiency and flexibility.