Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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rna-triggered conformational gating of guide rna activity

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toehold-gated guide RNA

RNA Element
Since 2024conditional activation of cas9 by endogenous or programmed rna inputsconditional activation of cas9 by endogenous rna inputsrna-triggered conformational gating of guide rna activitytoehold-mediated rna sensing

Toehold-gated guide RNA (thgRNA) is a synthetic riboregulatory guide RNA class that controls CRISPR/Cas9 activity in response to RNA inputs. Available evidence indicates that endogenous RNA transcripts can trigger thgRNA to activate Cas9 functions, supporting autonomous RNA-responsive control of genome-targeting activity.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
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