Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
8 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-8 of 8
Phage display is an assay and selection method used during engineering workflows for light-responsive protein tools. In the cited context, it is applied alongside computational protein design and high-throughput binding assays in development of LOV2-based optogenetic systems such as improved light-induced dimers.
our optimized prime editing strategy provides a highly efficient and versatile framework for genome engineering in vitro
LC-MS analysis of fittest binders is an assay method used with small combinatorial libraries of self-assembled proteomimetics (SAPs) to identify enriched target binders after affinity selection by liquid chromatography–mass spectrometry. In the cited SAP study, this workflow was applied in the context of target-directed selection from self-assembled PNA-peptide conjugate libraries.
We then use Tope-seq to screen epitope libraries against a model therapeutic candidate TCR.
Emerging targets such as B7-H3, Claudin 18.2, and MUC1, along with advancements in companion diagnostics, are reshaping the landscape of CAR-T therapy by enabling more precise patient selection and real-time therapeutic monitoring.
We also incorporate strategies for iterative biopanning and bioinformatic refinement to improve sensitivity and accuracy.
We also incorporate strategies for iterative biopanning and bioinformatic refinement to improve sensitivity and accuracy.
This review summarizes current research on innovative molecular approaches, including marker-assisted selection (MAS)... for soybean improvement.