Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
DsFbFP is a flavin-binding fluorescent protein from Dinoroseobacter shibae used as a genetically encoded photosensitizer. In a 2020 Biomolecules study, plasma-membrane-targeted DsFbFP in AR4-2J cells triggered persistent calcium oscillations upon light irradiation, consistent with photodynamic activation of cholecystokinin 1 receptor (CCK1R).
Vivid (VVD) is a blue-light-sensing light-oxygen-voltage (LOV) protein from the filamentous fungus Neurospora crassa. Upon illumination, its flavin cofactor forms a photoadduct that creates a stable light state, while VVD also exhibits light-dependent dimer-associated aggregation behavior and photosensitized self-oxidation.
Limited evidence for (1)O2 involvement in gene activation phenomena consists of deuterium oxide solvent effects, inhibition by (1)O2-quenchers, sensitization by porphyrins, chemical trapping methods, and comparative effects of photosensitizing dyes and thermolabile endoperoxides.