Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
Polyamidoamine (PAMAM) carriers spanning generations G0-G7 were characterized as polymeric nucleic acid delivery formulations for light-directed delivery in vitro using photochemical internalization (PCI). In an OHS EGFP osteosarcoma model, PAMAM G3-G7 supported efficient light-directed siRNA silencing, whereas EGFP mRNA up-regulation was not detected after EGFP mRNA/PAMAM transfection with or without PCI.
The upconversion nanoparticle-based siRNA nanocarrier is a nanoparticle delivery system reported to enable near-infrared-induced, spatiotemporally controlled gene silencing. It was demonstrated for spatially restricted GFP knockdown in 2D cell culture and 3D multicellular tumor spheroids.