Toolkit Items

Vivid-fused light-inducible recombinases are engineered Cre, Dre, and Flp variants generated by combining each site-specific recombinase with the fungal light-inducible protein Vivid. These constructs confer blue-light-driven recombinase activity and were used for light-induced genomic modification in live mouse brain, including under one-photon or two-photon illumination according to the provided summary.

The bicistronic expression cassette with GFP is a construct pattern used in Caenorhabditis elegans to co-express GFP as a reporter alongside conditional Channelrhodopsin-2 targeting components. In the cited study, GFP was included to identify the correct expression pattern during recombinase-based, intersectional single-neuron targeting.

Moreover, utilizing paper-based CRIBOS, which demonstrates remarkable portability and stability, we present a biological memory storage logic circuit device that can preserve DNA-based biological information for over 4 months with minimal resources, energy costs, and maintenance requirements.

BLADE, or Boolean Logic and Arithmetic through DNA Excision, is a platform that multiplexes light-inducible recombinases in mammalian cells. In the cited study, it was used as a DNA-excision-based control framework for Boolean logic and arithmetic operations and in a light-responsive recombinase context for cell patterning applications.

the development of mouse models characterised by cell-specific deletions of receptor subtype genes ... has greatly increased our understanding of the molecular mechanisms and neural substrates of nicotine addiction

Now, with the development of optogenetic tools and cell-type specific Cre-driver mouse lines, it has become possible to stimulate cholinergic axons from the basal forebrain (BF) and probe cholinergic synapses in the cortex for the first time.

The supplied web research summary explicitly identifies recombinase-driver rat lines as a platform/tool introduced by a primary paper and relevant to optogenetic application to dopamine-mediated reinforcement.

The Cre/loxP system is a tool for tissue-specific and time-specific knockout of target genes, which permits investigation of such genes.

The light-inducible split Cre recombinase is an optogenetic multi-component switch in which split Cre recombinase fragments are coupled to light-inducible dimerization modules to achieve inducible post-translational control of Cre activity. It was characterized by comprehensive screening of split sites across the Cre protein using a pooled, sequencing-based domain insertion profiling approach.

Explicitly supported across the anchor paper and the discovered atlas/resource papers as the recombinase system enabling intersectional targeting.

A cell-type-specific dual AAV system was employed, co-packaging a Cre-dependent Flpo plasmid and an Flpo-dependent enhanced yellow fluorescent protein (EYFP) plasmid.

In the last decade, there have been paradigm shifts in our understanding of the central melanocortin system as a result of the application of advanced new technologies, including Cre-LoxP transgenic mouse technology, pharmacogenetics and optogenetics.

This includes a comparative analysis of several mouse and rat lines expressing Cre or Flp recombinases under Tph2, Sert, or Pet1 promoters with a focus on specificity and recombination efficiency.

We present the cell-free recombinase-integrated Boolean output system (CRIBOS), a site-specific recombinase-based multiplex genetic circuit platform designed for cell-free environments.

FLP/FRT and CRISPR/Cas9 homology-directed repair (HDR) strategies, as well as their combinations, are currently the most effective for SSTI in maize.

This includes a comparative analysis of several mouse and rat lines expressing Cre or Flp recombinases under Tph2, Sert, or Pet1 promoters with a focus on specificity and recombination efficiency.

Primary INTRSECT paper introducing the multi-recombinase Boolean expression framework explicitly connected to reviewed intersectional AAV approaches and later extensions using Cre/Flpo logic.