Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
The TetR autorepression loop is a yeast gene-control construct pattern in which TetR represses both its own expression and a gene of interest, creating a negative autoregulatory circuit. In the reported implementation, induction is achieved with anhydrotetracycline to reversibly control protein dosage.
This tool is a light-mediated implementation of lac promoter-based gene expression that uses photocaged isopropyl β-D-thiogalactopyranoside (IPTG) as a synthetic inducer. In Corynebacterium glutamicum, it was applied for light-mediated optimization of lac promoter-driven expression and (+)-valencene biosynthesis.
we used two metabolite-inducible systems ... and TtgR from the Pseudomonas putida ttg operon, to assess their use as gene regulation platforms beyond reporter assays