Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
Visible-light-sensitive human OPN5 variants are engineered human neuropsin constructs in which Lys91 is replaced with neutral or acidic amino acids. This single-residue substitution substantially shifts hOPN5 spectral sensitivity from native UV responsiveness toward visible wavelengths, positioning these variants as candidate optogenetic tools.
We focused on human OPN5 (hOPN5) to attempt to identify amino acid(s) responsible for the UV sensitivity and to shift the spectral sensitivity to visible light... We applied site-directed mutagenesis to replace this residue with neutral (alanine) or acidic (glutamate or aspartate) amino acids... provide visible-light sensitive versions of this protein as a candidate for optogenetic applications.