Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP) is a red trans-synaptic marker built from split sfCherry3 and neuroligin-1-based linkage across synaptic partners. It was reported to enable multiplexed visualization of neuronal synapses in living Caenorhabditis elegans.
Split sfCherry3 variants are engineered split red fluorescent proteins derived from sfCherry through directed evolution. They were reported to show substantially enhanced overall brightness and to support visualization of endogenous proteins and neuronal synapses.