Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

7 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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split-enzyme reconstitution

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CIB1

Protein Domain
Since 2004Heterodimerizationlight-induced heterodimerizationOligomerizationprotein recruitment/localization controlreversible dissociation after light withdrawalsplit-enzyme reconstitutionStructural Characterization

As a tool component, CIB1 is most directly supported as the cryptochrome-interacting bHLH partner used with CRY2 to create blue-light-controlled protein association systems. In these systems, CIB1 is typically fused to localization, transcriptional, or enzymatic modules so that blue light drives CRY2–CIB1 binding and light withdrawal reverses the interaction.

CFBacMamMusHumTxRep
Ev 74Rep 54Pr 83

Light Activated BioID

Multi-Component Switch
Since 2022Heterodimerizationlight-induced heterodimerizationproximity-dependent biotinylationsplit-enzyme reconstitution

Light Activated BioID (LAB) is an optically controlled proximity-labeling system in which the two halves of split-TurboID are fused to the photodimerizing proteins CRY2 and CIB1. Blue light induces CRY2–CIB1 association, reconstituting split-TurboID and enabling proximity-dependent biotinylation of nearby proteins.

CFBacMamMusHumTxRep
Ev 44Rep 20Pr 49

mOptoT7

Multi-Component Switch
Since 2022light-induced heterodimerizationorthogonal transcriptionsplit-enzyme reconstitution

mOptoT7 is a mammalian optogenetic transcription system composed of a split T7 RNA polymerase fused to the blue-light-inducible nMag/pMag Magnets photodimerization system. Blue light drives reconstitution of the split polymerase to activate transcription from orthogonal T7 promoters in mammalian cells, and the system has been used to produce protein-coding mRNA, shRNA, and the Pepper RNA aptamer.

CFBacMamMusHumTxRep
Ev 40Rep 20Pr 49

split APEX peroxidase

Multi-Component Switch
Since 2018split-enzyme reconstitutionDirected Evolution

Split APEX peroxidase (sAPEX) is an engineered two-fragment version of APEX generated by directed evolution. It consists of a 200-amino acid N-terminal fragment (AP) and a 50-amino acid C-terminal fragment (EX) that can reconstitute peroxidase activity in mammalian cells.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 49

MagMboI

Multi-Component Switch
Since 2025Heterodimerizationlight-gated nuclease activationPhotocleavagesplit-enzyme reconstitutionComputational DesignStructural Characterization

MagMboI is a photoactivatable split version of the type II restriction endonuclease MboI configured as a multi-component switch for light-controlled nuclease function. The supplied evidence indicates that an optimized variant, MagMboI-plus, produced stronger genome rearrangement activity than the original MagMboI in Saccharomyces cerevisiae.

CFBacMamMusHumTxRep
Ev 40Rep 9Pr 37

split T7 RNA polymerase fused to blue-light-inducible Magnets

Multi-Component Switch
Since 2025blue-light controlriboregulatory digitizationsplit-enzyme reconstitutiontranslational repression

The split T7 RNA polymerase fused to blue-light-inducible Magnets is an optogenetic multi-component switch for light-controlled transcription. In the cited work, this system was enhanced by incorporation of a tet-controlled riboregulatory module to digitize the output of blue-light-regulated gene expression.

CFBacMamMusHumTxRep
Ev 28Rep 0Pr 37
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