Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

4 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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split-protein complementation

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split Cas9 logic gate

Multi-Component Switch
Since 2023Heterodimerizationlogic-gated transcriptional input integrationprotein splicing-mediated reconstitutionsplit-protein complementationComputational Design

The split Cas9 logic gate is a synthetic multi-component circuit that uses split Cas9 halves to sense biological events through conditional reconstitution of Cas9 activity. In the reported 2023 implementation, self-assembling inteins reconstituted split Cas9 and enabled reporter activation only when specified transcriptional or cell-state inputs were present.

CFBacMamMusHumTxRep
Ev 58Rep 9Pr 49
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