Toolkit Items

EGxxFP is a Cas9 reporter construct used in split-Cas9 synthetic circuits to convert successful Cas9 reconstitution into a fluorescent readout. In the cited 2023 Scientific Reports study, it reported cellular states and events including cancer epithelial origin, epithelial-to-mesenchymal transition, and cell-cell fusion.

This tool is a split luminescent enzyme construct engineered with a magneto-sensitive protein so that enzyme reconstitution is driven by a 50 mT magnetic stimulus. It was described as a component of a magneto-photonic gene circuit in mammalian cells for minimally invasive control of gene expression.

The intein-mediated split-Cas9 system is a multi-component CRISPR-Cas9 architecture in which Cas9 is divided into separate parts and reconstituted through split inteins. It was developed to bypass packaging limits while retaining nuclease activity comparable to wild-type Cas9, and a split-Cas9D10A version showed activity similar to Cas9D10A nickase.

The split and inducible adenine base editor is a multi-component adenine base editing system reported for precise in vivo base editing. The available evidence indicates that the editor is both split and inducible, but the supplied material does not specify its molecular architecture or induction modality.

CreLite is an optogenetically controlled Cre/loxP recombination system reported in developing zebrafish embryos. It uses split Cre recombinase halves fused to the red light-inducible partners PhyB and PIF6, so that 660 nm illumination in the presence of phycocyanobilin (PCB) restores Cre activity.

The light-inducible split Cre recombinase is an optogenetic multi-component switch in which split Cre recombinase fragments are coupled to light-inducible dimerization modules to achieve inducible post-translational control of Cre activity. It was characterized by comprehensive screening of split sites across the Cre protein using a pooled, sequencing-based domain insertion profiling approach.

PA-Cre2.0 is a photoactivatable split Cre recombinase in which Cre activity is reconstituted by light-induced CRY2-CIB1 dimerization. It enables light-dependent control of Cre-mediated recombination and has been functionally characterized in mammalian cells and rodent brain.

PhyB-CreC is the PhyB-fused C-terminal half of Cre recombinase in the CreLite optogenetic split-Cre system. In the presence of phycocyanobilin and 660 nm red light, it associates with PIF6-CreN to reconstitute Cre activity and drive Cre/loxP-dependent recombination in zebrafish embryos.

PIF6-CreN is the PIF6-fused N-terminal Cre fragment used in the CreLite optogenetic recombinase system. In the presence of phycocyanobilin (PCB), 660 nm red light induces association with PhyB-CreC, reconstituting Cre recombinase activity for light-controlled loxP recombination in developing zebrafish embryos.

Here, we present the CRISTAL (Control of RNA with Inducible SpliT CAs13 Orthologs and Exogenous Ligands) platform. CRISTAL is powered by a collection (10 total) of orthogonal split inducible Cas13 effectors that can be turned ON or OFF via small molecules in multiple cell types, providing precise temporal control.

The supplied web research summary states that the review spans photoactivatable genome editing and that the directly matching primary paper identifies the split Cas9 light-inducible system as paCas9 using pMag and nMag.