Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

2 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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state-selective protein-protein binding

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Am1_c0023g2

Protein Domain
Since 2016chromophore-dependent spectral tuninglight-dependent photoconversionstate-selective protein-protein binding

Am1_c0023g2 is a cyanobacteriochrome photosensory domain from Acaryochloris marina that covalently binds either phycocyanobilin or biliverdin with high binding efficiency. It has been used as the light-responsive target for engineered, state-selective binders that enable green-, orange-, red-, and far-red-controlled protein-protein interactions and transcriptional regulation in yeast.

CFBacMamMusHumTxRep
Ev 37Rep 20Pr 59

phage-display based approach

Engineering Method
Since 2019light-dependent control of protein-protein interactionsstate-selective protein-protein binding

This phage-display based approach was used to generate small monomeric binders that selectively recognize green-absorbing, red-absorbing, and far-red-absorbing photostates of the cyanobacteriochrome Am1_c0023g2 under specified chromophore conditions. The resulting binders enabled multicolor light-dependent control of protein-protein interactions in vitro and transcriptional control in yeast in vivo.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59
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