Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
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Translational titration is an application of genetic code expansion in Bacillus subtilis that modulates protein production at the level of translation. In the cited study, it was implemented within a broad and efficient noncanonical amino acid incorporation platform that also supported click-labelling and photo-crosslinking.
Genetic code expansion in Bacillus subtilis is a translation-engineering method for site-specific incorporation of non-standard amino acids into proteins in vivo. The reported system achieved broad and efficient incorporation of 20 distinct non-standard amino acids in B. subtilis using three families of genetic code expansion systems and two codon choices.