Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
6 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-6 of 6
C-terminal iLID fusion is a commonly used anchor configuration within the iLID optogenetic recruitment system. In the cited study, it functions as a baseline membrane-anchor design for light-controlled recruitment of components to subcellular locations, including micron-scale regions of the plasma membrane.
The Destruction Complex is a Wnt signal transduction protein assembly that processes and promotes degradation of β-catenin. A 2022 study reported that nucleation of this assembly on the centrosome accelerates β-catenin degradation and changes Wnt-dependent human embryonic stem cell fate outcomes.
Membrane-tethered CRY2 is a CRY2/CIB optical dimerization configuration in which CRY2 is localized at a membrane to control recruitment of CIB-linked partners with light. The reported application demonstrates that this arrangement is functional and may provide improved local control of protein interactions.
Optimized iLID membrane anchor configurations are alternative membrane-anchored fusion designs for the iLID optogenetic recruitment system that support light-gated recruitment of proteins to subcellular sites, including micron-scale regions of the plasma membrane. The reported purpose of these configurations is to provide greater control of subcellular protein localization across diverse cell biological applications.
p21-CRY2/CIB1 is a blue-light-responsive optogenetic p21 system built by fusing p21 to the cryptochrome 2/CIBN switch components. It was used to control p21 subcellular localization and nuclear function and increased the fraction of cells arrested in G1 phase.
The PhyB-based red/far-red optogenetic checkpoint control system is a multi-component optogenetic platform built around phytochrome B for rapid, reversible light control of protein localization and cell-cycle checkpoint regulation. In fission yeast engineered to produce phycocyanobilin, it enables red/far-red light-dependent recruitment to the plasma membrane, nucleus, and kinetochore, and includes an Opto-G2/M configuration that induces G2/M arrest under red light.