Toolkit Items

Dual synthetic pathway and dual-ligand microfluidic tissue patterning is a synNotch-based material-to-cell signaling strategy in which mammalian cells carrying two distinct synthetic pathways are cultured on surfaces microfluidically patterned with two synNotch ligands. In the cited 2023 study, this configuration generated tissues containing up to three distinct cell phenotypes in user-defined spatial arrangements.

Fibronectin-conjugated synNotch ligand display is a material-to-cell signaling construct in which synNotch ligands such as GFP are conjugated to cell-generated extracellular matrix proteins through genetic engineering of fibronectin produced by fibroblasts. The resulting ECM-presented ligand can activate synthetic Notch receptors and induce prescribed transcriptional programs in mammalian cells.

The gelatin-linked synNotch ligand hydrogel platform is a material-to-cell signaling system in which synNotch ligands are covalently attached to gelatin polymers by enzymatic conjugation or click chemistry. These ligand-functionalized hydrogels activate synthetic Notch receptors in mammalian cells cultured on or within the material, enabling prescribed transcriptional responses and spatial control of cell phenotypes.

Material-to-cell synNotch ligand platforms are engineered biomaterial and extracellular matrix systems that present synNotch ligands to mammalian cells. In the reported 2023 implementation, ligands were covalently incorporated into gelatin hydrogels or into cell-generated fibronectin-containing extracellular matrix to activate synthetic Notch receptors and induce programmed transcriptional outputs.

functioning as "training centers" to activate synthetic Notch (synNotch) CAR-T cells to produce CARs against a universal tumour antigen to exterminate neighboring tumour cells.