multi-level circuits
Construct PatternThese regulatory mechanisms, such as transcription and translation control, could be integrated into hybrid circuits termed "multi-level circuits".
CFBacMamMusHumTxRep
Ev 26Rep 9Pr 71
Toolkit Items
Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
▾Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 1
Mechanisms
Layer 2
Architectures
▾Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 2
Architectures
Layer 3
Components
▾Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Layer 3
Components
Technique Branch
Layer 1
Approaches
▾High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 1
Approaches
Layer 2
Methods
▾Concrete methods used to design, build, verify, or characterize engineered systems.
Layer 2
Methods
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transcriptional control
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thermal sonogenetics
Engineering MethodSince 2026focused ultrasound-mediated localized heatingheat-responsive promoter activationtranscriptional controlTranslation Control
Focused ultrasound (FUS) offers a non-invasive alternative capable of achieving localized and deep tissue heating, enabling precise activation of genetically engineered cells through heat-responsive promoters, a strategy termed thermal sonogenetics.
CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59
CRISPR-based transcriptional control
Engineering MethodEmerging synthetic biology tools, such as CRISPR-based transcriptional control, high-throughput screening, and machine learning-assisted promoter design, are enabling the creation of tunable, orthogonal promoters suited for complex multigene expression.
CFBacMamMusHumTxRep
incoherent feed-forward loop
Multi-Component SwitchWe perform RNA-sequencing on cells expressing an incoherent feed-forward loop (iFFL), a genetic circuit that enhances operational capacity.
CFBacMamMusHumTxRep
melanopsin-NFAT optogenetic transcription circuit
Multi-Component SwitchIn addition to neural activity, the ectopic expression of OPN4 has been reported to enable circuit control of the nuclear factor of activated T cells (NFAT) to enhance blood-glucose homeostasis in mice.
CFBacMamMusHumTxRep
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