Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

1 item matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

▾

Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

▾

Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

▾

Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

▾

High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

▾

Concrete methods used to design, build, verify, or characterize engineered systems.

Sort
transcriptional decoy sequestration of nf-κb

Showing 1-1 of 1

Loaditems
Page 1 / 1

caged NF-κB DNA decoy

RNA Element
Since 2011light-gated restoration of dna:dna hybridizationlight-gated restoration of transcription factor bindingPhotocleavagetranscriptional decoy sequestration of nf-κb

The caged NF-κB DNA decoy is a photoregulated oligonucleotide decoy in which photoremovable protecting groups are installed on nucleobases within an NF-κB decoy sequence. UV irradiation removes the caging groups, restores decoy hybridization and NF-κB binding activity, and enables light-controlled regulation of NF-κB-driven transcription in mammalian cells.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59
Page 1 / 1