Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
This tool is a light-mediated implementation of lac promoter-based gene expression that uses photocaged isopropyl β-D-thiogalactopyranoside (IPTG) as a synthetic inducer. In Corynebacterium glutamicum, it was applied for light-mediated optimization of lac promoter-driven expression and (+)-valencene biosynthesis.
PaX560-QacR is a light-mediated transcriptional regulation system that couples the caging-group-free photoactivatable dye PaX560 to the multidrug-binding transcriptional repressor QacR. Visible-light-driven traceless photoconversion generates a cationic dye that binds QacR, weakens repression, and activates transcription in vitro and in living cells.