Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

3 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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translational repression

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translational AND gates

Construct Pattern
Since 2021cas protein binding to 5'-utr rna motifstranslational activationtranslational repressiontranslation controlTranslation ControlComputational Design

Translational AND gates are artificial mammalian gene circuit elements created by interconnecting Cas-mediated translational switches. They implement combinatorial logic at the level of mRNA translation using Cas proteins that repress or activate transcripts bearing Cas-binding RNA motifs in the 5'-UTR, and a set of 60 such AND gates was reported.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71

tet-controlled riboregulatory module

RNA Element
Since 2025light-dependent relief of translational repressionregulatory layer integrationtranslational repressiontranslation controlTranslation ControlComputational Design

The tet-controlled riboregulatory module is a synthetic RNA regulatory element incorporated into a blue-light split T7 RNA polymerase-Magnets optogenetic system. In the supplied evidence, it functions as an added regulatory layer intended to improve circuit behavior in light-controlled gene expression.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59

split T7 RNA polymerase fused to blue-light-inducible Magnets

Multi-Component Switch
Since 2025blue-light controlriboregulatory digitizationsplit-enzyme reconstitutiontranslational repression

The split T7 RNA polymerase fused to blue-light-inducible Magnets is an optogenetic multi-component switch for light-controlled transcription. In the cited work, this system was enhanced by incorporation of a tet-controlled riboregulatory module to digitize the output of blue-light-regulated gene expression.

CFBacMamMusHumTxRep
Ev 28Rep 0Pr 37
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