Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
10 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-10 of 10
carefully matching biophysical properties of actuators and indicators can permit unambiguous excitation with a single wavelength in a so-called single-beam all-optical experiment
We will focus on two outstanding studies of 2012 that took advantage of two-photon microscopy to increase the spatial resolution
improved optogenetic tools can now be easily combined with two-photon excitation, allowing light stimulation of single cells in vivo
propose a new strategy for a single-beam two-photon excitation experiment to monitor activity minimizing cross-activation with the actuators
Functional nucleic acid (FNA) probes are nucleic-acid-based biosensing probes used in environmental monitoring, food analysis, clinical diagnosis, and biological imaging. The cited evidence specifically emphasizes two-photon-based FNA probes as fluorescence biosensing formats with improved optical performance over one-photon-based FNA probes in biomedical sensing.
Multi-photon excitation (MPE) microscopy plays a growing role among microscopical techniques utilized for studying biological matter.
Two-photon-based functional nucleic acid probes are functional nucleic acid biosensing and imaging probes that operate through two-photon excitation or two-photon activation. Reported examples are positioned for biosensing and biomedical imaging, with claimed performance advantages over conventional one-photon functional nucleic acid probes.
Nowadays, 2PE and MPE microscopes are expected to increase their impact in areas such biotechnology, neurobiology, embryology, tissue engineering, materials science.
Today, intravital microscopy with two-photon excitation microscopes (2P-IVM) is the mainstay technique for observing intercellular cross-talks in situ, unraveling cellular and molecular mechanisms in the context of their spatiotemporal dynamics.
The supplied web research summary explicitly names two-photon in vivo imaging as a method used for chronic longitudinal monitoring of retrosplenial cortex activity-linked reporter expression in related primary studies.