Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
4 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-4 of 4
recent advances in optogenetic actuators, genetically encoded calcium and voltage indicators, and patterned photostimulation have transformed in vitro research, enabling all-optical interrogation of synaptic plasticity, functional connectivity, and emergent network dynamics.
Chemogenetically driven repositioning of lysosomes is an experimental perturbation used to causally alter lysosome localization and test how lysosome positioning regulates endoplasmic reticulum remodeling. In the cited 2020 Science Advances study, chemo- and optogenetically driven lysosome repositioning was used to validate a causal link between lysosome movement and ER network organization.
DLC1 (Deleted in Liver Cancer 1) is a Rho-specific GTPase-activating protein domain-level regulator of Rho signaling. In the cited 2023 study, DLC1 was implicated in dynamic association with focal adhesions during optogenetically induced reinforcement and relaxation, linking its GAP function to spatial control of contractility.
This tool is an optogenetic actuator used to control Rho activity with local and reversible effects on cellular contractility. In the cited 2023 study, it was applied to probe DLC1-dependent regulation of Rho signaling at focal adhesions and the plasma membrane.