Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
A NIMPLY B gates are compressed two-input mixed-phenotype transcriptional logic operations reported in a 2023 ACS Synthetic Biology study on performance prediction of fundamental transcriptional programs. The study indicates that their behavior can be modeled and predicted from experimentally characterized single-input logical operations and associated metrology.
Engineered BUFFER logical operations are single-input transcriptional logic elements described in a collection of network-capable genetic programs. In the cited study, the BUFFER operation was implemented as a repressor-based logical operation and experimentally characterized as part of a framework for predictive design of more complex transcriptional circuits.
Engineered BUFFER (repressor) and engineered NOT (antirepressor) logical operations are single-input transcriptional logic elements developed as network-capable components for synthetic gene programs. They were experimentally characterized and used as foundational parts for predictive modeling of more complex compressed transcriptional logic circuits.