Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
14 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-14 of 14
N282 is the N-terminal 282-amino-acid fragment of Arabidopsis COP1, derived from mutant cDNAs and expressed in transgenic plants as a dominant-negative protein domain. Its overexpression induces phenotypes and dark-grown genome expression patterns resembling cop1 loss-of-function and white-light responses, consistent with interference with endogenous COP1 repression of photomorphogenesis.
H2B-tKR is a chromatin-targeted phototoxic construct in which histone H2B is fused to tandem KillerRed to control cell division with green light. Upon illumination, it induces nuclear damage-associated mitotic defects, including chromosome nondisjunction during metaphase, and can transiently block proliferation.
The nCas9ERT2 fusion protein, consisting of Cas9 and an estrogen receptor flanked by two nuclear localization signals, is usually located in the cytoplasm and efficiently translocated into nuclei upon 4-hydroxytamoxifen (4-OHT) treatment in cultured cells or embryos.
Antisense RNA is an RNA-based regulatory tool reported in tobacco to restore fertility in genetically engineered male sterile plants. The available evidence indicates a functional phenotype in this plant system, but does not specify the target gene, antisense construct design, or molecular implementation.
The GUS-NC80 fusion protein is a transgenic plant construct in which β-glucuronidase is fused to the 80-residue NC80 motif from Arabidopsis CRY2. In the cited study, this fusion was constitutively active despite being unphosphorylated, supporting that NC80 is sufficient to confer CRY2 physiological function when derepressed.
stimulating a transgenically expressed Gq-GPCR (MrgA1) mimics the physiological Ca2+ signaling underlying gliotransmitter release
The Arabidopsis phyA:GFP fusion protein is a fluorescent phytochrome A construct expressed in phyA-deficient Arabidopsis and used to monitor light-regulated intracellular behavior. In Arabidopsis, the construct complements the phyA mutant phenotype and reports light-dependent subcellular partitioning, including nuclear import and cytosolic sequestered area formation.
we introduced the channelrhodopsin variant ChR2 XXM2.0, which exhibits high light sensitivity and enhanced Ca2+ conductance
The CRY2 C-terminal tail from Arabidopsis CRY2 contains an 80-residue NC80 motif that is sufficient to confer CRY2 physiological function. Evidence from transgenic plant studies indicates that this region participates in blue light-responsive regulation through phosphorylation-linked derepression of NC80.
The system is based on the fluorescent protein DRONPA-s, which can be switched on and off repeatedly by illumination with different light qualities. Using transgenic 35S::DRONPA-s Arabidopsis thaliana and a confocal microscope it was possible to activate DRONPA-s fluorescence in selected cells of the root meristem.
GUS-CRY2 is a fusion protein comprising Arabidopsis cryptochrome 2 (CRY2) and the reporter enzyme GUS. In transgenic plants, it localizes to the nucleus and functions in vivo as a blue light-responsive photoreceptor that mediates inhibition of hypocotyl elongation.
There are two CALI approaches that use either transgenic tags with chemical photosensitizers, or genetically encoded fluorescent protein fusions.
Some of the highlights in the past 6 years include the use of ... inducible transgenes to experimentally modulate FGF signaling
OptoR2 is a light-activated FGFR2 variant engineered to selectively activate signaling by the major fibroblast growth factor receptor in keratinocytes. Upon illumination, it induces FGFR signaling and, in OptoR2-expressing HEK293T cells, promotes migration and proliferation with temporal precision.