Techniques

Measuring the activity or performance of an engineered part in a biological context.

In silico design of protein sequences, structures, or circuits using algorithms or machine learning.

Determining 3D structure via X-ray crystallography, cryo-EM, or NMR.

Experimental selection from a library to enrich for variants with desired properties.

Iterative mutagenesis and selection to evolve improved biological parts.

Confirming construct identity by Sanger or next-generation sequencing.