Source 1primary paper2017Journal of Clinical Investigation
Toolkit/AAV9-DIO-hM3Dq
AAV9-DIO-hM3Dq
Also known as: AAV9-DIO-hM3Dq chemogenetic construct, hM3Dq
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Explicitly supported related items include VGAT-Cre, hM3Dq/AAV9-DIO-hM3Dq chemogenetic constructs, optogenetic depolarization of GABAergic DRG neurons.
Usefulness & Problems
Why this is useful
This Cre-dependent chemogenetic construct is used to depolarize GABAergic dorsal root ganglion neurons in vivo. In the paper context, such depolarization reduced acute and chronic peripherally induced nociception.; chemogenetic depolarization of GABAergic dorsal root ganglion neurons in vivo
Source:
This Cre-dependent chemogenetic construct is used to depolarize GABAergic dorsal root ganglion neurons in vivo. In the paper context, such depolarization reduced acute and chronic peripherally induced nociception.
Source:
chemogenetic depolarization of GABAergic dorsal root ganglion neurons in vivo
Problem solved
It provides a way to causally test whether activating GABAergic DRG neurons suppresses nociceptive transmission.; enables targeted activation of GABAergic DRG neurons to test their effect on nociception
Source:
It provides a way to causally test whether activating GABAergic DRG neurons suppresses nociceptive transmission.
Source:
enables targeted activation of GABAergic DRG neurons to test their effect on nociception
Problem links
enables targeted activation of GABAergic DRG neurons to test their effect on nociception
LiteratureIt provides a way to causally test whether activating GABAergic DRG neurons suppresses nociceptive transmission.
Source:
It provides a way to causally test whether activating GABAergic DRG neurons suppresses nociceptive transmission.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: payload burdenimplementation constraint: spectral hardware requirementoperating role: sensor
Use requires a Cre driver such as VGAT-Cre and AAV-based delivery to dorsal root ganglia. The abstract supports in vivo chemogenetic depolarization but does not specify additional execution details.; requires VGAT-Cre or equivalent Cre driver; requires AAV delivery to dorsal root ganglion neurons
The provided evidence does not show that it is a therapeutic delivery platform by itself or that it resolves all mechanisms of pain modulation.; requires Cre-dependent targeting context
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Chemogenetic or optogenetic depolarization of GABAergic dorsal root ganglion neurons in vivo reduced acute and chronic peripherally induced nociception.
We also demonstrated that chemogenetic or optogenetic depolarization of GABAergic dorsal root ganglion neurons in vivo reduced acute and chronic peripherally induced nociception.
Approval Evidence
1 source1 linked approval claimfirst-pass slug aav9-dio-hm3dq
Explicitly supported related items include VGAT-Cre, hM3Dq/AAV9-DIO-hM3Dq chemogenetic constructs, optogenetic depolarization of GABAergic DRG neurons.
Source:
application effectsupports
Chemogenetic or optogenetic depolarization of GABAergic dorsal root ganglion neurons in vivo reduced acute and chronic peripherally induced nociception.
We also demonstrated that chemogenetic or optogenetic depolarization of GABAergic dorsal root ganglion neurons in vivo reduced acute and chronic peripherally induced nociception.
Source:
Comparisons
Source-stated alternatives
The source also mentions optogenetic depolarization of GABAergic DRG neurons as an alternative activation approach.
Source:
The source also mentions optogenetic depolarization of GABAergic DRG neurons as an alternative activation approach.
Source-backed strengths
supports in vivo manipulation of a defined neuronal population
Source:
supports in vivo manipulation of a defined neuronal population
Compared with optogenetic
The source also mentions optogenetic depolarization of GABAergic DRG neurons as an alternative activation approach.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports in vivo manipulation of a defined neuronal population.
Relative tradeoffs: requires Cre-dependent targeting context.
Source:
The source also mentions optogenetic depolarization of GABAergic DRG neurons as an alternative activation approach.
Ranked Citations
- 1.