Source 1primary paper2013Nature Neuroscience
Toolkit/optogenetic
optogenetic
Also known as: optogenetic
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This entry describes an optogenetic engineering method applied to identify a rapid eye movement sleep modulatory circuit in the hypothalamus. The available evidence supports only that light-based circuit interrogation was used in this neurobiological context.
Usefulness & Problems
Why this is useful
The method is useful for probing how defined neural circuits contribute to rapid eye movement sleep regulation in the hypothalamus. The supplied evidence indicates utility for circuit identification, but does not provide technical performance details.
Problem solved
It addresses the problem of identifying a hypothalamic circuit that modulates rapid eye movement sleep. The evidence does not specify the targeted cell types, opsins, or stimulation paradigms used to solve this problem.
Problem links
Need precise spatiotemporal control with light input
DerivedThis entry describes an optogenetic engineering method applied to identify a rapid eye movement sleep modulatory circuit in the hypothalamus. The available evidence supports only that light-based circuit interrogation was used in this neurobiological context.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
gain-of-function perturbationlight-dependent neural circuit perturbationloss-of-function perturbationTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuator
The method involves light as the input modality and an optogenetic approach for neural circuit interrogation. No further implementation details, such as construct design, delivery method, species, or hardware parameters, are provided in the evidence.
The evidence is limited to the study focus and does not report the molecular actuator, wavelength, expression strategy, or behavioral assay details. Independent replication and generalizability beyond this reported application are not established from the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2016Neurotherapeutics
Ranked Claims
Circuit-specific manipulation tools are discussed as useful for identifying neural circuits that mediate behavior and for repair of neural connections.
Optogenetic and chemogenetic approaches are reviewed as genetic techniques for gain- and loss-of-function within specific neural circuits.
The study uses an optogenetic approach to identify a rapid eye movement sleep modulatory circuit in the hypothalamus.
The study uses an optogenetic approach to identify a rapid eye movement sleep modulatory circuit in the hypothalamus.
The study uses an optogenetic approach to identify a rapid eye movement sleep modulatory circuit in the hypothalamus.
The study uses an optogenetic approach to identify a rapid eye movement sleep modulatory circuit in the hypothalamus.
The study uses an optogenetic approach to identify a rapid eye movement sleep modulatory circuit in the hypothalamus.
The study uses an optogenetic approach to identify a rapid eye movement sleep modulatory circuit in the hypothalamus.
The study uses an optogenetic approach to identify a rapid eye movement sleep modulatory circuit in the hypothalamus.
Approval Evidence
1 source1 linked approval claimfirst-pass slug optogenetic
Optogenetic identification of a rapid eye movement sleep modulatory circuit in the hypothalamus
Source:
study focussupports
The study uses an optogenetic approach to identify a rapid eye movement sleep modulatory circuit in the hypothalamus.
Source:
Comparisons
Source-backed strengths
A key strength supported by the evidence is that optogenetic perturbation enabled identification of a rapid eye movement sleep modulatory circuit in the hypothalamus. No quantitative validation, temporal precision metrics, or comparative advantages are described in the supplied material.
Compared with mMORp
optogenetic and mMORp address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with optogenetic probes
optogenetic and optogenetic probes address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with organoid fusion
optogenetic and organoid fusion address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.
- 2.