Source 1primary paper2017Nature Communications
Toolkit/aptazyme-embedded guide RNAs
aptazyme-embedded guide RNAs
Also known as: aptazyme-embedded gRNAs, guide RNAs
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Aptazyme-embedded guide RNAs are engineered CRISPR guide RNA constructs that confer ligand-responsive control over CRISPR outputs. Reported functions include ligand-responsive genome editing and ligand-responsive transcriptional activation.
Usefulness & Problems
Why this is useful
These constructs are useful for coupling chemical input signals to CRISPR activity at the guide RNA level. The available evidence indicates utility for conditional control of both genome editing and transcriptional activation.
Source:
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Problem solved
Aptazyme-embedded guide RNAs address the problem of making CRISPR outputs responsive to ligand input rather than constitutively active. Specifically, they provide a reported route to chemically regulated genome editing and transcriptional activation.
Problem links
Need controllable genome or transcript editing
DerivedAptazyme-embedded guide RNAs are engineered CRISPR guide RNA constructs that confer ligand-responsive control over CRISPR outputs. Reported functions include ligand-responsive genome editing and ligand-responsive transcriptional activation.
Need tighter control over gene expression timing or amplitude
DerivedAptazyme-embedded guide RNAs are engineered CRISPR guide RNA constructs that confer ligand-responsive control over CRISPR outputs. Reported functions include ligand-responsive genome editing and ligand-responsive transcriptional activation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Mechanisms
ligand-responsive rna regulationTechniques
No technique tags yet.
Target processes
editingtranscriptionInput: Chemical
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: regulator
The tool consists of guide RNAs engineered to embed an aptazyme, indicating an RNA-level construct design strategy. The provided evidence does not report sequence design rules, required cofactors, delivery format, or expression context.
The supplied evidence does not specify the ligands, aptazyme architecture, guide RNA insertion site, CRISPR effector, dynamic range, leakiness, or host system. Independent replication and comparative benchmarking are not established from the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive transcriptional activation.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive transcriptional activation.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive transcriptional activation.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive transcriptional activation.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive transcriptional activation.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive transcriptional activation.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Aptazyme-embedded guide RNAs enable ligand-responsive transcriptional activation.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Approval Evidence
1 source2 linked approval claimsfirst-pass slug aptazyme-embedded-guide-rnas
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Source:
functional capabilitysupports
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Source:
functional capabilitysupports
Aptazyme-embedded guide RNAs enable ligand-responsive transcriptional activation.
Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Source:
Comparisons
Source-backed strengths
The cited study reports two distinct functional outputs from the same general tool class: ligand-responsive genome editing and ligand-responsive transcriptional activation. This indicates that the approach can be applied across more than one CRISPR-mediated process.
Compared with ligand-activated and ligand-deactivated sgRNAs
aptazyme-embedded guide RNAs and ligand-activated and ligand-deactivated sgRNAs address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; same primary input modality: chemical
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with riboswitch-based amino acid sensors
aptazyme-embedded guide RNAs and riboswitch-based amino acid sensors address a similar problem space because they share editing, transcription.
Shared frame: same top-level item type; shared target processes: editing, transcription
Strengths here: looks easier to implement in practice.
Compared with small regulatory RNAs
aptazyme-embedded guide RNAs and small regulatory RNAs address a similar problem space because they share editing, transcription.
Shared frame: same top-level item type; shared target processes: editing, transcription
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.