Source 1review2017Bioresource Technology
Toolkit/small regulatory RNAs
small regulatory RNAs
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
To address current limitations of metabolic engineering, this article gives insights on recent systems metabolic engineering approaches based on functional tools and method such as genome reduction, amino acid sensors based on transcriptional regulators and riboswitches, CRISPR interference, small regulatory RNAs...
Usefulness & Problems
Why this is useful
Small regulatory RNAs are presented as functional tools for systems metabolic engineering of amino acid producing strains. They imply RNA-level control of gene expression in the production host.; post-transcriptional regulation in systems metabolic engineering; engineering amino acid producing strains
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Small regulatory RNAs are presented as functional tools for systems metabolic engineering of amino acid producing strains. They imply RNA-level control of gene expression in the production host.
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post-transcriptional regulation in systems metabolic engineering
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engineering amino acid producing strains
Problem solved
The review includes small regulatory RNAs as one route to address current limitations in metabolic engineering.; addresses current limitations of metabolic engineering
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The review includes small regulatory RNAs as one route to address current limitations in metabolic engineering.
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addresses current limitations of metabolic engineering
Problem links
addresses current limitations of metabolic engineering
LiteratureThe review includes small regulatory RNAs as one route to address current limitations in metabolic engineering.
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The review includes small regulatory RNAs as one route to address current limitations in metabolic engineering.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Mechanisms
rna-level regulation of gene expressionTechniques
No technique tags yet.
Target processes
editingtranscriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Implementation requires expression of small regulatory RNAs in the engineered strain. The abstract does not specify scaffold designs, helper proteins, or target-selection methods.; requires small regulatory RNA design and expression in the host
The abstract does not describe their specificity limits, burden, or comparison to CRISPR interference in practice.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Recent systems metabolic engineering approaches to address current limitations of metabolic engineering include genome reduction, amino acid sensors based on transcriptional regulators and riboswitches, CRISPR interference, small regulatory RNAs, DNA scaffolding, and optogenetic control.
Approval Evidence
1 source1 linked approval claimfirst-pass slug small-regulatory-rnas
To address current limitations of metabolic engineering, this article gives insights on recent systems metabolic engineering approaches based on functional tools and method such as genome reduction, amino acid sensors based on transcriptional regulators and riboswitches, CRISPR interference, small regulatory RNAs...
Source:
toolkit overviewsupports
Recent systems metabolic engineering approaches to address current limitations of metabolic engineering include genome reduction, amino acid sensors based on transcriptional regulators and riboswitches, CRISPR interference, small regulatory RNAs, DNA scaffolding, and optogenetic control.
Source:
Comparisons
Source-stated alternatives
The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
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The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
Source-backed strengths
highlighted as a recent functional tool
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highlighted as a recent functional tool
Compared with CRISPR/Cas9
The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: highlighted as a recent functional tool.
Source:
The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
Compared with CRISPR/Cas9 system
The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: highlighted as a recent functional tool.
Source:
The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
Compared with CRISPR interference
The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: highlighted as a recent functional tool.
Source:
The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
Compared with DNA scaffolding
The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: highlighted as a recent functional tool.
Source:
The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
Compared with optogenetic
The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: highlighted as a recent functional tool.
Source:
The abstract mentions CRISPR interference, amino acid sensors, DNA scaffolding, genome reduction, and optogenetic control as nearby alternatives.
Ranked Citations
- 1.