Source 1primary paper2021Nature Communications
Toolkit/artificial differentiation system
artificial differentiation system
Also known as: artificial differentiation strategy, light tunable differentiation system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The artificial differentiation system is a light-tunable construct pattern in budding yeast based on optogenetically driven genetic rewiring. It is designed to generate stable microbial consortia with user-defined composition in space and time from a single strain and supports dynamic control of consortium composition in continuous culture.
Usefulness & Problems
Why this is useful
This system is useful for building stable yeast consortia with custom functionalities without relying on conventional co-culturing alone. The reported light tunability enables temporal and spatial control over subpopulation composition and supports extended control in continuous cultures.
Source:
Here, we present an artificial differentiation system in budding yeast capable of generating stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and in time based on optogenetically-driven genetic rewiring.
Problem solved
It addresses the difficulty of generating stable microbial consortia with finely tuned compositions, a limitation noted for standard co-culturing approaches. The system specifically provides a way to derive controlled consortium structure from a single starting strain.
Problem links
Need precise spatiotemporal control with light input
DerivedThe artificial differentiation system is a light-tunable construct pattern in budding yeast based on optogenetically driven genetic rewiring. It is designed to generate stable microbial consortia with user-defined composition in space and time from a single strain and supports dynamic control of consortium composition in continuous culture.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuator
The system was implemented in budding yeast and is described as light tunable and based on optogenetically driven genetic rewiring. The available evidence does not provide construct design details, cofactors, expression components, or delivery and cultivation parameters beyond use in continuous cultures.
The supplied evidence does not specify the underlying optogenetic photoreceptor, illumination wavelength, circuit architecture, or quantitative performance metrics. Independent replication beyond the cited 2021 study is not provided in the evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The artificial differentiation system in budding yeast can generate stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and time.
Here, we present an artificial differentiation system in budding yeast capable of generating stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and in time based on optogenetically-driven genetic rewiring.
The artificial differentiation system in budding yeast can generate stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and time.
Here, we present an artificial differentiation system in budding yeast capable of generating stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and in time based on optogenetically-driven genetic rewiring.
The artificial differentiation system in budding yeast can generate stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and time.
Here, we present an artificial differentiation system in budding yeast capable of generating stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and in time based on optogenetically-driven genetic rewiring.
The artificial differentiation system in budding yeast can generate stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and time.
Here, we present an artificial differentiation system in budding yeast capable of generating stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and in time based on optogenetically-driven genetic rewiring.
The artificial differentiation system in budding yeast can generate stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and time.
Here, we present an artificial differentiation system in budding yeast capable of generating stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and in time based on optogenetically-driven genetic rewiring.
The artificial differentiation system in budding yeast can generate stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and time.
Here, we present an artificial differentiation system in budding yeast capable of generating stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and in time based on optogenetically-driven genetic rewiring.
The artificial differentiation system in budding yeast can generate stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and time.
Here, we present an artificial differentiation system in budding yeast capable of generating stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and in time based on optogenetically-driven genetic rewiring.
Co-culturing approaches remain limited in their ability to generate stable consortia with finely tuned compositions.
Co-culturing approaches, the preferred mode of generating a consortium, remain limited in their ability to give rise to stable consortia having finely tuned compositions.
Co-culturing approaches remain limited in their ability to generate stable consortia with finely tuned compositions.
Co-culturing approaches, the preferred mode of generating a consortium, remain limited in their ability to give rise to stable consortia having finely tuned compositions.
Co-culturing approaches remain limited in their ability to generate stable consortia with finely tuned compositions.
Co-culturing approaches, the preferred mode of generating a consortium, remain limited in their ability to give rise to stable consortia having finely tuned compositions.
Co-culturing approaches remain limited in their ability to generate stable consortia with finely tuned compositions.
Co-culturing approaches, the preferred mode of generating a consortium, remain limited in their ability to give rise to stable consortia having finely tuned compositions.
Co-culturing approaches remain limited in their ability to generate stable consortia with finely tuned compositions.
Co-culturing approaches, the preferred mode of generating a consortium, remain limited in their ability to give rise to stable consortia having finely tuned compositions.
Co-culturing approaches remain limited in their ability to generate stable consortia with finely tuned compositions.
Co-culturing approaches, the preferred mode of generating a consortium, remain limited in their ability to give rise to stable consortia having finely tuned compositions.
Co-culturing approaches remain limited in their ability to generate stable consortia with finely tuned compositions.
Co-culturing approaches, the preferred mode of generating a consortium, remain limited in their ability to give rise to stable consortia having finely tuned compositions.
The system enables dynamic control of consortia composition in continuous cultures for extended periods.
Owing to fast, reproducible, and light-tunable dynamics, our system enables dynamic control of consortia composition in continuous cultures for extended periods.
The system enables dynamic control of consortia composition in continuous cultures for extended periods.
Owing to fast, reproducible, and light-tunable dynamics, our system enables dynamic control of consortia composition in continuous cultures for extended periods.
The system enables dynamic control of consortia composition in continuous cultures for extended periods.
Owing to fast, reproducible, and light-tunable dynamics, our system enables dynamic control of consortia composition in continuous cultures for extended periods.
The system enables dynamic control of consortia composition in continuous cultures for extended periods.
Owing to fast, reproducible, and light-tunable dynamics, our system enables dynamic control of consortia composition in continuous cultures for extended periods.
The system enables dynamic control of consortia composition in continuous cultures for extended periods.
Owing to fast, reproducible, and light-tunable dynamics, our system enables dynamic control of consortia composition in continuous cultures for extended periods.
The system enables dynamic control of consortia composition in continuous cultures for extended periods.
Owing to fast, reproducible, and light-tunable dynamics, our system enables dynamic control of consortia composition in continuous cultures for extended periods.
The system enables dynamic control of consortia composition in continuous cultures for extended periods.
Owing to fast, reproducible, and light-tunable dynamics, our system enables dynamic control of consortia composition in continuous cultures for extended periods.
The system can be extended to generate consortia with multiple subpopulations.
We further demonstrate that our system can be extended in a straightforward manner to give rise to consortia with multiple subpopulations.
The system can be extended to generate consortia with multiple subpopulations.
We further demonstrate that our system can be extended in a straightforward manner to give rise to consortia with multiple subpopulations.
The system can be extended to generate consortia with multiple subpopulations.
We further demonstrate that our system can be extended in a straightforward manner to give rise to consortia with multiple subpopulations.
The system can be extended to generate consortia with multiple subpopulations.
We further demonstrate that our system can be extended in a straightforward manner to give rise to consortia with multiple subpopulations.
The system can be extended to generate consortia with multiple subpopulations.
We further demonstrate that our system can be extended in a straightforward manner to give rise to consortia with multiple subpopulations.
The system can be extended to generate consortia with multiple subpopulations.
We further demonstrate that our system can be extended in a straightforward manner to give rise to consortia with multiple subpopulations.
The system can be extended to generate consortia with multiple subpopulations.
We further demonstrate that our system can be extended in a straightforward manner to give rise to consortia with multiple subpopulations.
The paper reports a light tunable differentiation system for creating and controlling consortia in yeast.
A light tunable differentiation system for the creation and control of consortia in yeast
Section: title
The paper reports a light tunable differentiation system for creating and controlling consortia in yeast.
A light tunable differentiation system for the creation and control of consortia in yeast
Section: title
The paper reports a light tunable differentiation system for creating and controlling consortia in yeast.
A light tunable differentiation system for the creation and control of consortia in yeast
Section: title
The paper reports a light tunable differentiation system for creating and controlling consortia in yeast.
A light tunable differentiation system for the creation and control of consortia in yeast
Section: title
The paper reports a light tunable differentiation system for creating and controlling consortia in yeast.
A light tunable differentiation system for the creation and control of consortia in yeast
Section: title
The paper reports a light tunable differentiation system for creating and controlling consortia in yeast.
A light tunable differentiation system for the creation and control of consortia in yeast
Section: title
Approval Evidence
1 source5 linked approval claimsfirst-pass slugs artificial-differentiation-system, light-tunable-differentiation-system
A light tunable differentiation system for the creation and control of consortia in yeast
Source:
Here, we present an artificial differentiation system in budding yeast ... based on optogenetically-driven genetic rewiring.
Source:
capabilitysupports
The artificial differentiation system in budding yeast can generate stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and time.
Here, we present an artificial differentiation system in budding yeast capable of generating stable microbial consortia with custom functionalities from a single strain at user-defined composition in space and in time based on optogenetically-driven genetic rewiring.
Source:
comparative limitationsupports
Co-culturing approaches remain limited in their ability to generate stable consortia with finely tuned compositions.
Co-culturing approaches, the preferred mode of generating a consortium, remain limited in their ability to give rise to stable consortia having finely tuned compositions.
Source:
dynamic controlsupports
The system enables dynamic control of consortia composition in continuous cultures for extended periods.
Owing to fast, reproducible, and light-tunable dynamics, our system enables dynamic control of consortia composition in continuous cultures for extended periods.
Source:
extensibilitysupports
The system can be extended to generate consortia with multiple subpopulations.
We further demonstrate that our system can be extended in a straightforward manner to give rise to consortia with multiple subpopulations.
Source:
system applicationsupports
The paper reports a light tunable differentiation system for creating and controlling consortia in yeast.
A light tunable differentiation system for the creation and control of consortia in yeast
Source:
Comparisons
Source-backed strengths
Reported strengths include generation of stable microbial consortia, user-defined control of composition in space and time, and dynamic regulation of consortium composition over extended periods in continuous culture. The design is also described as capable of producing consortia with custom functionalities from a single strain.
Source:
Co-culturing approaches, the preferred mode of generating a consortium, remain limited in their ability to give rise to stable consortia having finely tuned compositions.
Compared with optogenetic actuator
artificial differentiation system and optogenetic actuator address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: optogenetic control; same primary input modality: light
Compared with split recombinases
artificial differentiation system and split recombinases address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: optogenetic control; same primary input modality: light
Compared with TRIM21-nanobody chimeras
artificial differentiation system and TRIM21-nanobody chimeras address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: optogenetic control; same primary input modality: light
Ranked Citations
- 1.