Source 1review2022PubMed
Toolkit/split recombinases
split recombinases
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Split recombinases are recombinase enzymes partitioned into inactive fragments that can be reactivated in light-controlled inducible recombination systems. The supplied evidence indicates that optogenetic switches mediate reactivation of the split fragments to control recombination.
Usefulness & Problems
Why this is useful
This construct pattern is useful for placing recombination under optical control. The evidence supports its use in optogenetic inducible recombination systems, but does not provide quantitative performance or application-specific outcomes.
Problem solved
It addresses the problem of making recombinase activity inducible by light rather than constitutively active. The supplied evidence specifically supports light-mediated reactivation of split recombinases through optogenetic switches.
Problem links
Need conditional recombination or state switching
DerivedSplit recombinases are recombinase enzymes partitioned into inactive fragments that can be reactivated in light-controlled inducible recombination systems. The supplied evidence indicates that optogenetic switches mediate reactivation of the split fragments to control recombination.
Need precise spatiotemporal control with light input
DerivedSplit recombinases are recombinase enzymes partitioned into inactive fragments that can be reactivated in light-controlled inducible recombination systems. The supplied evidence indicates that optogenetic switches mediate reactivation of the split fragments to control recombination.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: split
Implementation requires a recombinase to be split into inactive fragments and coupled to an optogenetic switching strategy that enables light-dependent reactivation. The supplied evidence does not specify construct architecture, cofactors, expression systems, or delivery methods.
The evidence is limited to a general statement that optogenetic switches reactivate split recombinases in inducible recombination systems. It does not identify specific recombinases, split sites, light-sensing modules, wavelengths, recombination efficiencies, or validation contexts.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Optogenetic inducible recombination systems rely on reactivation of split recombinases mediated by optogenetic switches.
While optogenetic inducible recombination systems rely on reactivations of split recombinases that mediated by optogenetic switches.
Optogenetic inducible recombination systems rely on reactivation of split recombinases mediated by optogenetic switches.
While optogenetic inducible recombination systems rely on reactivations of split recombinases that mediated by optogenetic switches.
Optogenetic inducible recombination systems rely on reactivation of split recombinases mediated by optogenetic switches.
While optogenetic inducible recombination systems rely on reactivations of split recombinases that mediated by optogenetic switches.
Optogenetic inducible recombination systems rely on reactivation of split recombinases mediated by optogenetic switches.
While optogenetic inducible recombination systems rely on reactivations of split recombinases that mediated by optogenetic switches.
Optogenetic inducible recombination systems rely on reactivation of split recombinases mediated by optogenetic switches.
While optogenetic inducible recombination systems rely on reactivations of split recombinases that mediated by optogenetic switches.
Optogenetic inducible recombination systems rely on reactivation of split recombinases mediated by optogenetic switches.
While optogenetic inducible recombination systems rely on reactivations of split recombinases that mediated by optogenetic switches.
Optogenetic inducible recombination systems rely on reactivation of split recombinases mediated by optogenetic switches.
While optogenetic inducible recombination systems rely on reactivations of split recombinases that mediated by optogenetic switches.
Approval Evidence
1 source1 linked approval claimfirst-pass slug split-recombinases
While optogenetic inducible recombination systems rely on reactivations of split recombinases that mediated by optogenetic switches.
Source:
mechanism summarysupports
Optogenetic inducible recombination systems rely on reactivation of split recombinases mediated by optogenetic switches.
While optogenetic inducible recombination systems rely on reactivations of split recombinases that mediated by optogenetic switches.
Source:
Comparisons
Source-backed strengths
A key strength supported by the evidence is conditional control of recombination through optogenetic reactivation of an otherwise split, inactive recombinase. No additional strengths such as kinetics, dynamic range, wavelength specificity, or organismal validation are provided in the supplied evidence.
split recombinases and modular light-controlled skeletal muscle-powered bioactuator address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Compared with Opto-Casp8-V2
split recombinases and Opto-Casp8-V2 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Compared with pcVP16
split recombinases and pcVP16 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Ranked Citations
- 1.