Source 1review2007Microbiology and Molecular Biology Reviews
Toolkit/Autodisplay
Autodisplay
Also known as: autodisplay system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The autodisplay system was developed on the basis of the natural Escherichia coli autotransporter protein AIDA-I (adhesin involved in diffuse adherence). Autodisplay has been used for the surface display of random peptide libraries to successfully screen for novel enzyme inhibitors. The autodisplay system was also used for the surface display of functional enzymes... Autodisplay of epitopes on the surface of attenuated Salmonella carriers has also provided a novel way to induce immune protection after oral vaccination.
Usefulness & Problems
Why this is useful
Autodisplay is an autotransporter-based construct pattern for exporting recombinant passenger proteins to the surface of gram-negative bacteria. The review describes its use for peptide library display, enzyme display, and epitope display.; bacterial surface display of recombinant proteins; display of random peptide libraries; screening for enzyme inhibitors; whole-cell biocatalysis; surface presentation of vaccine epitopes
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Autodisplay is an autotransporter-based construct pattern for exporting recombinant passenger proteins to the surface of gram-negative bacteria. The review describes its use for peptide library display, enzyme display, and epitope display.
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bacterial surface display of recombinant proteins
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display of random peptide libraries
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screening for enzyme inhibitors
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whole-cell biocatalysis
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surface presentation of vaccine epitopes
Problem solved
It provides a simple route to surface presentation of diverse recombinant proteins and libraries on intact bacterial cells. This supports screening, biocatalysis, and vaccine-style antigen display applications.; exports heterologous passenger proteins to the bacterial surface using an autotransporter architecture; enables cell-surface presentation of libraries and functional proteins without disrupting bacterial viability
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It provides a simple route to surface presentation of diverse recombinant proteins and libraries on intact bacterial cells. This supports screening, biocatalysis, and vaccine-style antigen display applications.
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exports heterologous passenger proteins to the bacterial surface using an autotransporter architecture
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enables cell-surface presentation of libraries and functional proteins without disrupting bacterial viability
Problem links
enables cell-surface presentation of libraries and functional proteins without disrupting bacterial viability
LiteratureIt provides a simple route to surface presentation of diverse recombinant proteins and libraries on intact bacterial cells. This supports screening, biocatalysis, and vaccine-style antigen display applications.
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It provides a simple route to surface presentation of diverse recombinant proteins and libraries on intact bacterial cells. This supports screening, biocatalysis, and vaccine-style antigen display applications.
exports heterologous passenger proteins to the bacterial surface using an autotransporter architecture
LiteratureIt provides a simple route to surface presentation of diverse recombinant proteins and libraries on intact bacterial cells. This supports screening, biocatalysis, and vaccine-style antigen display applications.
Source:
It provides a simple route to surface presentation of diverse recombinant proteins and libraries on intact bacterial cells. This supports screening, biocatalysis, and vaccine-style antigen display applications.
Published Workflows
Objective: Use autodisplay to engineer bacterial cell surfaces for discovery, catalysis, or immunization by presenting recombinant peptides, enzymes, or epitopes.
Why it works: The review states that passengers can be transported when fused in frame between an N-terminal signal peptide and a C-terminal beta-barrel translocation domain, enabling intact bacterial cells to present large numbers of recombinant molecules on their surface for screening or functional use.
autotransporter-mediated outer membrane translocationcell-surface presentation of recombinant passengerspost-transport oligomerizationpost-transport incorporation of inorganic prosthetic groupssurface displayrandom peptide library screeningwhole-cell biocatalysisoral vaccine carrier design
Stages
- 1.Surface display library generation(library_build)
The abstract states that autodisplay was used for surface display of random peptide libraries, establishing the starting material for inhibitor screening.
Selection: Build random peptide libraries on the bacterial surface using autodisplay.
- 2.Inhibitor screening on displayed peptide libraries(broad_screen)
This stage uses the displayed library to discover inhibitory peptides or binders against enzyme targets.
Selection: Screen displayed random peptide libraries to identify novel enzyme inhibitors.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
Oligomerizationouter membrane autotransporter-mediated translocationpost-translocation prosthetic group incorporationsurface localizationTarget processes
localizationrecombinationselectionImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: regulator
The abstract states that the passenger coding sequence must be inserted in frame between an N-terminal signal peptide and a C-terminal beta-barrel translocation domain. It is described as being developed from the E. coli autotransporter AIDA-I.; uses gram-negative bacterial autotransporter secretion context; requires an N-terminal signal peptide; requires a C-terminal beta-barrel outer membrane translocation unit; requires in-frame fusion of the passenger coding sequence with transport elements
The abstract does not define the full range of passengers that fail in autodisplay or compare failure modes against other display systems. It only states a general architectural requirement for in-frame fusion to transport elements.; requires the coding sequence to be inserted in frame between an N-terminal signal peptide and a C-terminal beta-barrel translocation domain
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Autodisplay has been used for surface display of functional enzymes including esterases, oxidoreductases, and electron transfer proteins.
The autodisplay system was also used for the surface display of functional enzymes, including esterases, oxidoreductases, and electron transfer proteins.
Autodisplay has been used to display random peptide libraries for screening novel enzyme inhibitors.
Autodisplay has been used for the surface display of random peptide libraries to successfully screen for novel enzyme inhibitors.
Autodisplay of epitopes on attenuated Salmonella carriers has been used as an oral vaccination strategy to induce immune protection.
Autodisplay of epitopes on the surface of attenuated Salmonella carriers has also provided a novel way to induce immune protection after oral vaccination.
Whole E. coli cells displaying enzymes via autodisplay have been used to synthesize rare chiral organic compounds.
Whole E. coli cells displaying enzymes have been utilized to efficiently synthesize industrially important rare organic compounds with specific chirality.
Autodisplay facilitates export of more than 100,000 recombinant molecules per single cell.
Autodisplay facilitates the export of more than 100,000 recombinant molecules per single cell
exported recombinant molecules per cell 100000
Autodisplay permits oligomerization of subunits on the cell surface and incorporation of inorganic prosthetic groups after transport of apoproteins without disturbing bacterial integrity or viability.
permits the oligomerization of subunits on the cell surface as well as the incorporation of inorganic prosthetic groups after transport of apoproteins onto the bacterial surface without disturbing bacterial integrity or viability
The autodisplay system was developed on the basis of the natural Escherichia coli autotransporter protein AIDA-I.
the autodisplay system was developed on the basis of the natural Escherichia coli autotransporter protein AIDA-I
Autodisplay transports recombinant or native passenger proteins when their coding sequence is inserted in frame between an N-terminal signal peptide and a C-terminal beta-barrel outer membrane translocation domain.
Proteins are transported, independent of their nature as recombinant or native passengers, as long as the coding nucleotide sequence is inserted in frame between those of an N-terminal signal peptide and a C-terminal domain, referred to as the beta-barrel of the outer membrane translocation unit.
Approval Evidence
1 source8 linked approval claimsfirst-pass slug autodisplay
The autodisplay system was developed on the basis of the natural Escherichia coli autotransporter protein AIDA-I (adhesin involved in diffuse adherence). Autodisplay has been used for the surface display of random peptide libraries to successfully screen for novel enzyme inhibitors. The autodisplay system was also used for the surface display of functional enzymes... Autodisplay of epitopes on the surface of attenuated Salmonella carriers has also provided a novel way to induce immune protection after oral vaccination.
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application scopesupports
Autodisplay has been used for surface display of functional enzymes including esterases, oxidoreductases, and electron transfer proteins.
The autodisplay system was also used for the surface display of functional enzymes, including esterases, oxidoreductases, and electron transfer proteins.
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application scopesupports
Autodisplay has been used to display random peptide libraries for screening novel enzyme inhibitors.
Autodisplay has been used for the surface display of random peptide libraries to successfully screen for novel enzyme inhibitors.
Source:
application scopesupports
Autodisplay of epitopes on attenuated Salmonella carriers has been used as an oral vaccination strategy to induce immune protection.
Autodisplay of epitopes on the surface of attenuated Salmonella carriers has also provided a novel way to induce immune protection after oral vaccination.
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application scopesupports
Whole E. coli cells displaying enzymes via autodisplay have been used to synthesize rare chiral organic compounds.
Whole E. coli cells displaying enzymes have been utilized to efficiently synthesize industrially important rare organic compounds with specific chirality.
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capacity summarysupports
Autodisplay facilitates export of more than 100,000 recombinant molecules per single cell.
Autodisplay facilitates the export of more than 100,000 recombinant molecules per single cell
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functional capabilitysupports
Autodisplay permits oligomerization of subunits on the cell surface and incorporation of inorganic prosthetic groups after transport of apoproteins without disturbing bacterial integrity or viability.
permits the oligomerization of subunits on the cell surface as well as the incorporation of inorganic prosthetic groups after transport of apoproteins onto the bacterial surface without disturbing bacterial integrity or viability
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historical originsupports
The autodisplay system was developed on the basis of the natural Escherichia coli autotransporter protein AIDA-I.
the autodisplay system was developed on the basis of the natural Escherichia coli autotransporter protein AIDA-I
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mechanism summarysupports
Autodisplay transports recombinant or native passenger proteins when their coding sequence is inserted in frame between an N-terminal signal peptide and a C-terminal beta-barrel outer membrane translocation domain.
Proteins are transported, independent of their nature as recombinant or native passengers, as long as the coding nucleotide sequence is inserted in frame between those of an N-terminal signal peptide and a C-terminal domain, referred to as the beta-barrel of the outer membrane translocation unit.
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Comparisons
Source-stated alternatives
The review places autodisplay within the broader autotransporter pathway and notes earlier IgA1 protease-based experiments. No detailed head-to-head alternative platform comparison is given in the abstract.
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The review places autodisplay within the broader autotransporter pathway and notes earlier IgA1 protease-based experiments. No detailed head-to-head alternative platform comparison is given in the abstract.
Source-backed strengths
described as a simple secretion/display solution; can transport recombinant or native passengers when inserted in frame between an N-terminal signal peptide and C-terminal beta-barrel domain; facilitates export of more than 100,000 recombinant molecules per single cell; permits oligomerization of subunits on the cell surface; permits incorporation of inorganic prosthetic groups after transport of apoproteins onto the bacterial surface without disturbing bacterial integrity or viability
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described as a simple secretion/display solution
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can transport recombinant or native passengers when inserted in frame between an N-terminal signal peptide and C-terminal beta-barrel domain
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facilitates export of more than 100,000 recombinant molecules per single cell
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permits oligomerization of subunits on the cell surface
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permits incorporation of inorganic prosthetic groups after transport of apoproteins onto the bacterial surface without disturbing bacterial integrity or viability
Compared with cell-free system
Autodisplay and cell-free system address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Compared with luciferin-luciferase pair
Autodisplay and luciferin-luciferase pair address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Strengths here: looks easier to implement in practice.
Compared with synthetic promoters
Autodisplay and synthetic promoters address a similar problem space because they share localization, recombination, selection.
Shared frame: same top-level item type; shared target processes: localization, recombination, selection
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.