Source 1primary paper2023
Toolkit/automated 96-well microplate illumination and measurement
automated 96-well microplate illumination and measurement
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Automated 96-well microplate illumination and measurement is an assay method for high-throughput optogenetic characterization of cultures under controlled light input. In the cited Saccharomyces cerevisiae workflow, it supported construction and characterization of split transcription factors containing cryptochrome and Enhanced Magnet light-sensitive dimerizers.
Usefulness & Problems
Why this is useful
This method is useful for scaling optogenetic experiments by combining automated illumination and measurement in a 96-well format with laboratory automation. The cited study used it to enable high-throughput characterization of yeast optogenetic split transcription factors.
Source:
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Problem solved
It addresses the low-throughput nature of manually illuminating and measuring optogenetic cultures during tool development. In the cited workflow, it helped solve the need for efficient construction and screening of light-responsive transcription factor designs in Saccharomyces cerevisiae.
Problem links
Need precise spatiotemporal control with light input
DerivedThis assay method automates illumination and measurement of cultures in a 96-well microplate format for high-throughput characterization. In the cited yeast optogenetics workflow, it was used to support construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
Need tighter control over gene expression timing or amplitude
DerivedThis assay method automates illumination and measurement of cultures in a 96-well microplate format for high-throughput characterization. In the cited yeast optogenetics workflow, it was used to support construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
light-controlled dimerizationlight-controlled dimerizationtranscriptional regulationtranscriptional regulationTechniques
Functional AssayTarget processes
transcriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensorswitch architecture: split
The method was implemented for cultures in a 96-well microplate format with automated illumination and measurement. In the cited application, it was integrated with laboratory automation and a modular cloning scheme in Saccharomyces cerevisiae to build and test split transcription factors using cryptochrome and Enhanced Magnet dimerizers.
The supplied evidence does not report detailed hardware specifications, illumination wavelengths, temporal precision, or quantitative assay performance metrics for the plate-based system. Validation is described in the context of a yeast optogenetics workflow, so broader applicability across organisms or assay classes is not established here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
Approval Evidence
1 source1 linked approval claimfirst-pass slug automated-96-well-microplate-illumination-and-measurement
automate illumination and measurement of cultures in a 96-well microplate format for high-throughput characterization
Source:
capabilitysupports
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Source:
Comparisons
Source-backed strengths
The main demonstrated strength is high-throughput characterization in a 96-well microplate format with automated illumination and measurement. In the associated workflow, laboratory automation plus modular cloning enabled construction and characterization of split transcription factors, and an optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
Source:
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
Compared with HY5
automated 96-well microplate illumination and measurement and HY5 address a similar problem space because they share transcription.
Shared frame: shared target processes: transcription; shared mechanisms: transcriptional regulation; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with light-inducible transcriptional effectors
automated 96-well microplate illumination and measurement and light-inducible transcriptional effectors address a similar problem space because they share transcription.
Shared frame: shared target processes: transcription; shared mechanisms: transcriptional regulation; same primary input modality: light
Strengths here: looks easier to implement in practice.
Compared with open-source microplate reader
automated 96-well microplate illumination and measurement and open-source microplate reader address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; same primary input modality: light
Ranked Citations
- 1.