Toolkit/open-source microplate reader

open-source microplate reader

Assay Method·Research·Since 2018

Also known as: open-source plate reader

Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

The open-source microplate reader is a low-cost, open-source assay platform for automated plate-based measurements. It was designed, constructed, validated, and benchmarked to support full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of assay protocols.

Usefulness & Problems

Why this is useful

This platform is useful because it combines standard microplate readouts with in situ light delivery for optogenetic experiments in a single automated instrument. The source literature also demonstrates its use for steady-state reporter measurements in bacterial quorum sensing and for flavin photocycling kinetics of a LOV-domain photoreceptor used in optogenetic transcriptional activation.

Source:

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.

Source:

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

Source:

we here report the design, construction, validation, and benchmarking of an open-source microplate reader

Problem solved

It addresses the need for a low-cost, open-source microplate reader that can perform automated absorbance and fluorescence assays while also delivering optogenetic stimulation. The reported demonstrations indicate that it supports both conventional reporter-gene measurements and photoreceptor kinetic measurements within plate-based workflows.

Source:

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.

Problem links

Need better screening or enrichment leverage

Derived

The open-source microplate reader is a low-cost assay platform designed, constructed, validated, and benchmarked for automated plate-based measurements. It supports full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of assay protocols.

Need conditional recombination or state switching

Derived

The open-source microplate reader is a low-cost assay platform designed, constructed, validated, and benchmarked for automated plate-based measurements. It supports full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of assay protocols.

Need precise spatiotemporal control with light input

Derived

The open-source microplate reader is a low-cost assay platform designed, constructed, validated, and benchmarked for automated plate-based measurements. It supports full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of assay protocols.

Need tighter control over gene expression timing or amplitude

Derived

The open-source microplate reader is a low-cost assay platform designed, constructed, validated, and benchmarked for automated plate-based measurements. It supports full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of assay protocols.

Taxonomy & Function

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor

The platform includes stand-alone touch screen programming for automated assay protocols and supports in situ optical stimulation during plate measurements. The evidence specifically mentions use in bacterial reporter assays and in kinetic measurements of a LOV-domain photoreceptor, but it does not provide further construct, hardware, or calibration details here.

The provided evidence does not report quantitative performance metrics such as sensitivity, dynamic range, throughput, or wavelength-specific limits. Independent replication is not described in the supplied evidence, and validation examples appear limited to the applications reported in the original study.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 2application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 3application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 4application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 5application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 6application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 7application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 8application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 9application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 10application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 11application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 12application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 13application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 14application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 15application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 16application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 17application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 18application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 19application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 20application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 21application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 22application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 23application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 24application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 25application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 26application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 27application demonstrationsupports2018Source 1needs review

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.
Claim 28capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 29capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 30capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 31capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 32capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 33capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 34capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 35capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 36capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 37capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 38capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 39capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 40capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 41capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 42capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 43capabilitysupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 44costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 45costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 46costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 47costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 48costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 49costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 50costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 51costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 52costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 53costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 54costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 55costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 56costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 57costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 58costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 59costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 60costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 61costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 62costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 63costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 64costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 65costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 66costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 67costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 68costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 69costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 70costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 71costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 72costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 73costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 74costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 75costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 76costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 77costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 78costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 79costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 80costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 81costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 82costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 83costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 84costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 85costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs less than $3500
system cost 3500 USD
Claim 86costsupports2018Source 1needs review

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers
system cost 3500 USD
Claim 87detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 88detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 89detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 90detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 91detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 92detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 93detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 94detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 95detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 96detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 97detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 98detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 99detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 100detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 101detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 102detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM
fluorescence detection limit 10 nM
Claim 103detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 104detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 105detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 106detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 107detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 108detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 109detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 110detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 111detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 112detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 113detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 114detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 115detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 116detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 117detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 118detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 119detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 120detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 121detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 122detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 123detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 124detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 125detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 126detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 127detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 128detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 129detection limitsupports2018Source 1needs review

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration
fluorescence detection limit 10 nanomolar
Claim 130documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 131documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 132documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 133documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 134documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 135documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 136documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 137documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 138documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 139documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 140documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 141documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 142documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 143documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 144documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 145documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 146documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 147documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 148documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 149documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 150documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 151documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 152documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 153documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 154documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 155documentationsupports2018Source 1needs review

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 156documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 157documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 158documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 159documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 160documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 161documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 162documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 163documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 164documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 165documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 166documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 167documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 168documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 169documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 170documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 171documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 172documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 173documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 174documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 175documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 176documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 177documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 178documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 179documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 180documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 181documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 182documentation availabilitysupports2018Source 1needs review

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.
Claim 183feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 184feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 185feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 186feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 187feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 188feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 189feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 190feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 191feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 192feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 193feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 194feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 195feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 196feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 197feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 198feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 199feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 200feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 201feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 202feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 203feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 204feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 205feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 206feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 207feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 208feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 209feature descriptionsupports2018Source 1needs review

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.
Claim 210tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 211tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 212tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 213tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 214tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 215tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 216tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 217tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 218tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 219tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 220tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 221tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 222tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 223tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 224tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader
Claim 225tool descriptionsupports2018Source 1needs review

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader

Approval Evidence

1 source10 linked approval claimsfirst-pass slug open-source-microplate-reader
we here report the design, construction, validation, and benchmarking of an open-source microplate reader

Source:

application demonstrationsupports

The open-source microplate reader was functionally demonstrated by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing and by flavin photocycling kinetic measurements of a LOV domain photoreceptor used for optogenetic transcriptional activation.

Functional capabilities were demonstrated in context of synthetic biology, optogenetics, and photosensory biology: by steady-state measurements of ligand-induced reporter gene expression in a model of bacterial quorum sensing, and by flavin photocycling kinetic measurements of a LOV (light-oxygen-voltage) domain photoreceptor used for optogenetic transcriptional activation.

Source:

capabilitysupports

The open-source microplate reader features full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

Source:

costsupports

The total system cost is less than $3500.

The total system costs <$3500, a fraction of the cost of commercial plate readers

Source:

costsupports

The total system cost is less than $3500.

The total system costs less than $3500

Source:

detection limitsupports

The open-source microplate reader can detect fluorescence of common dyes at concentrations as low as approximately 10 nM.

can detect the fluorescence of common dyes at concentrations as low as ∼10 nM

Source:

detection limitsupports

The open-source microplate reader can detect fluorescence of common dyes down to approximately 10 nanomolar concentration.

can detect the fluorescence of common dyes down to ∼10 nanomolar concentration

Source:

documentationsupports

The paper provides fully detailed guides for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.

Source:

documentation availabilitysupports

Fully detailed guides are provided for assembling the device and automating it using a custom Python-based API.

Fully detailed guides for assembling the device and automating it using the custom Python-based API (Application Program Interface) are provided.

Source:

feature descriptionsupports

The open-source microplate reader features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols.

Source:

tool descriptionsupports

The paper reports the design, construction, validation, and benchmarking of an open-source microplate reader.

we here report the design, construction, validation, and benchmarking of an open-source microplate reader

Source:

Comparisons

Source-backed strengths

Reported strengths include full-spectrum absorbance detection, fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen control of automated protocols. The instrument was not only built but also validated and benchmarked, and its functionality was demonstrated in both ligand-induced reporter gene expression assays and LOV-domain flavin photocycling measurements.

open-source microplate reader and droplet microfluidic platform address a similar problem space because they share recombination, selection.

Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light

open-source microplate reader and fluorescence recovery after photobleaching address a similar problem space because they share recombination, selection.

Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light

open-source microplate reader and light-switchable transcription factors address a similar problem space because they share recombination, selection, transcription.

Shared frame: shared target processes: recombination, selection, transcription; same primary input modality: light

Strengths here: looks easier to implement in practice.

Ranked Citations

  1. 1.
    StructuralSource 1Biochemistry2018Claim 23Claim 23Claim 27

    Seeded from load plan for claim c5. Extracted from this source document.