Source 1primary paper2016Advanced Optical Materials
Toolkit/azobenzene-containing crosslinkers carrying reactive maleimide groups
azobenzene-containing crosslinkers carrying reactive maleimide groups
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Azobenzene-containing crosslinkers carrying reactive maleimide groups are used to photocontrol the conformation and, hence, activity of proteins.
Usefulness & Problems
Why this is useful
These azobenzene-based crosslinkers are used to photocontrol protein conformation, which in turn modulates protein activity.; photocontrol of protein conformation; photocontrol of protein activity
Source:
These azobenzene-based crosslinkers are used to photocontrol protein conformation, which in turn modulates protein activity.
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photocontrol of protein conformation
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photocontrol of protein activity
Problem solved
They solve the problem of remotely controlling protein behavior with light by coupling a photoswitch to protein conformation.; enables light-dependent control of protein structure and function
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They solve the problem of remotely controlling protein behavior with light by coupling a photoswitch to protein conformation.
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enables light-dependent control of protein structure and function
Problem links
enables light-dependent control of protein structure and function
LiteratureThey solve the problem of remotely controlling protein behavior with light by coupling a photoswitch to protein conformation.
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They solve the problem of remotely controlling protein behavior with light by coupling a photoswitch to protein conformation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuator
The abstract supports the need for reactive maleimide-bearing crosslinkers, proteins to be modified, and light to drive switching.; requires reactive maleimide groups; requires protein-compatible crosslinking context; requires light exposure for switching
Needs compatible illumination hardware and optical access. Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
A near-infrared rhodamine dye prepared by photoirradiation is used as a fluorescent probe in cell imaging.
a near-infrared rhodamine dye, prepared by photoirradiation and used as a fluorescent probe in cell imaging
Fast photochromic imidazole dimers are used as the basis of a real-time dynamic hologram of a realistic 3D object.
a real-time dynamic hologram of a realistic 3D object based on fast photochromic imidazole dimers
Azobenzene-containing crosslinkers with reactive maleimide groups are used to photocontrol protein conformation and thereby protein activity.
Azobenzene-containing crosslinkers carrying reactive maleimide groups are used to photocontrol the conformation and, hence, activity of proteins.
Approval Evidence
1 source1 linked approval claimfirst-pass slug azobenzene-containing-crosslinkers-carrying-reactive-maleimide-groups
Azobenzene-containing crosslinkers carrying reactive maleimide groups are used to photocontrol the conformation and, hence, activity of proteins.
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mechanism applicationsupports
Azobenzene-containing crosslinkers with reactive maleimide groups are used to photocontrol protein conformation and thereby protein activity.
Azobenzene-containing crosslinkers carrying reactive maleimide groups are used to photocontrol the conformation and, hence, activity of proteins.
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Comparisons
Source-stated alternatives
The abstract contrasts this protein-control approach with other photochromic systems such as diarylethenes and spiropyrans used in materials and multifunctional switches.
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The abstract contrasts this protein-control approach with other photochromic systems such as diarylethenes and spiropyrans used in materials and multifunctional switches.
Source-backed strengths
links conformational control to activity control through light
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links conformational control to activity control through light
Compared with mMORp
azobenzene-containing crosslinkers carrying reactive maleimide groups and mMORp address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with optogenetic probes
azobenzene-containing crosslinkers carrying reactive maleimide groups and optogenetic probes address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with organoid fusion
azobenzene-containing crosslinkers carrying reactive maleimide groups and organoid fusion address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.