Source 1primary paper2020Journal of Synchrotron Radiation
Toolkit/B21
B21
Also known as: B21 SAXS beamline, Beamline B21
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
B21 is a small-angle X-ray scattering (SAXS) beamline with a bending magnet source in the 3 GeV storage ring at the Diamond Light Source Ltd synchrotron in the UK.
Usefulness & Problems
Why this is useful
B21 is a high-throughput SAXS beamline for measuring biological macromolecules at Diamond Light Source. It delivers X-rays to an in-vacuum detector and supports automated loading of solution samples.; high-throughput SAXS measurements; measuring biological macromolecules; automated loading of solution samples
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B21 is a high-throughput SAXS beamline for measuring biological macromolecules at Diamond Light Source. It delivers X-rays to an in-vacuum detector and supports automated loading of solution samples.
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high-throughput SAXS measurements
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measuring biological macromolecules
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automated loading of solution samples
Problem solved
It enables automated SAXS measurements with low background across a useful q-range for biological macromolecules. This supports efficient handling of many solution samples.; provides automated high-throughput SAXS data collection for solution samples; supports measurement of a wide range of biological macromolecules with low instrument background
Source:
It enables automated SAXS measurements with low background across a useful q-range for biological macromolecules. This supports efficient handling of many solution samples.
Source:
provides automated high-throughput SAXS data collection for solution samples
Source:
supports measurement of a wide range of biological macromolecules with low instrument background
Problem links
provides automated high-throughput SAXS data collection for solution samples
LiteratureIt enables automated SAXS measurements with low background across a useful q-range for biological macromolecules. This supports efficient handling of many solution samples.
Source:
It enables automated SAXS measurements with low background across a useful q-range for biological macromolecules. This supports efficient handling of many solution samples.
supports measurement of a wide range of biological macromolecules with low instrument background
LiteratureIt enables automated SAXS measurements with low background across a useful q-range for biological macromolecules. This supports efficient handling of many solution samples.
Source:
It enables automated SAXS measurements with low background across a useful q-range for biological macromolecules. This supports efficient handling of many solution samples.
Published Workflows
Objective: Provide high-throughput SAXS measurements for a wide range of biological macromolecules at Diamond Light Source.
Why it works: The beamline combines high photon flux, a small focal spot, low instrument background, and automated sample loading for solution samples, while retaining manual loading options for viscous or solid materials.
X-ray scattering measurement of biological macromoleculesautomated sample loadingHPLC-assisted sample handlingmanual custom-cell loading for non-solution materials
Stages
- 1.Automated solution sample loading(functional_characterization)
This stage enables a high level of automation for solution-sample SAXS measurements.
Selection: Solution samples are loaded using HPLC and a liquid-handling robot into a temperature-controlled in-vacuum sample cell.
- 2.Manual loading of viscous or solid materials(functional_characterization)
This stage extends the beamline to sample types not described as compatible with the automated solution-loading setup.
Selection: Viscous or solid materials are loaded manually using custom sample cells.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
small-angle x-ray scatteringTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Use of B21 requires synchrotron beamline access and its associated instrumentation, including the monochromator, focusing optic, detector, HPLC system, liquid-handling robot, and sample cell. Manual custom sample cells are used for some viscous or solid materials.; requires access to the Diamond Light Source synchrotron beamline infrastructure; automated solution loading depends on a high-performance liquid chromatography system, liquid-handling robot, and temperature-controlled in-vacuum sample cell
The abstract does not describe downstream SAXS data analysis or structural interpretation software. It also indicates that some non-solution materials require manual rather than automated loading.; manual loading is used for viscous or solid materials rather than the automated solution-loading mode
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
B21 is a high-throughput SAXS beamline at Diamond Light Source.
B21 has a default scattering vector range of 0.0026 to 0.34 Å^-1 and low instrument background, making it convenient for measuring a wide range of biological macromolecules.
scattering vector range 0.0026 to 0.34 Å^-1
The B21 beamline has run a full user programme since 2013.
user programme start 2013
B21 delivers 2 × 10^12 photons per second to a 34 × 40 µm focal spot at the in-vacuum Eiger 4M detector.
focal spot size 34 × 40 µm (FWHM)photon flux 2000000000000 photons per second
B21 can also measure viscous or solid materials by manual loading using custom sample cells.
An HPLC system and liquid-handling robot enable highly automated loading of solution samples into a temperature-controlled in-vacuum sample cell on B21.
Approval Evidence
1 source6 linked approval claimsfirst-pass slug b21
B21 is a small-angle X-ray scattering (SAXS) beamline with a bending magnet source in the 3 GeV storage ring at the Diamond Light Source Ltd synchrotron in the UK.
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capabilitysupports
B21 is a high-throughput SAXS beamline at Diamond Light Source.
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measurement rangesupports
B21 has a default scattering vector range of 0.0026 to 0.34 Å^-1 and low instrument background, making it convenient for measuring a wide range of biological macromolecules.
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operational statussupports
The B21 beamline has run a full user programme since 2013.
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performancesupports
B21 delivers 2 × 10^12 photons per second to a 34 × 40 µm focal spot at the in-vacuum Eiger 4M detector.
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sample compatibilitysupports
B21 can also measure viscous or solid materials by manual loading using custom sample cells.
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workflow capabilitysupports
An HPLC system and liquid-handling robot enable highly automated loading of solution samples into a temperature-controlled in-vacuum sample cell on B21.
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Comparisons
Source-stated alternatives
The abstract does not name alternative beamlines or competing SAXS platforms.
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The abstract does not name alternative beamlines or competing SAXS platforms.
Source-backed strengths
high photon flux; small focal spot; high level of automation for solution sample loading; supports both automated solution loading and manual loading of viscous or solid materials; wide default scattering vector range
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high photon flux
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small focal spot
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high level of automation for solution sample loading
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supports both automated solution loading and manual loading of viscous or solid materials
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wide default scattering vector range
Compared with small-angle X-ray scattering
The abstract does not name alternative beamlines or competing SAXS platforms.
Shared frame: source-stated alternative in extracted literature
Strengths here: high photon flux; small focal spot; high level of automation for solution sample loading.
Relative tradeoffs: manual loading is used for viscous or solid materials rather than the automated solution-loading mode.
Source:
The abstract does not name alternative beamlines or competing SAXS platforms.
Ranked Citations
- 1.